Extracellular lactase (beta-d-galactosidase, EC 3.2.1.23) was prepared as an ethanol precipitate from a culture of Fusarium moniliforme grown on whey. The enzyme functioned optimally at pH 3.8 to 5.0 and at 50 to 60 degrees C on both o-nitrophenyl-beta-d-galactopyranoside (ONPG) and lactose. The activation energy of the enzymic hydrolysis of ONPG and lactose in the range of 20 to 55 degrees C w...

Abstract This study evaluated the lactose hydrolysis by immobilized ?-galactosidase, which was produced Kluyveromyces marxianus using porungo cheese whey as substrate. Initially, yeast cultivated in medium at 30 °C and 200 rpm, showing a maximal ?-galactosidase production of 14.19 U mL-1. The crude extract obtained used to evaluate enzymatic solution. optimal pH temperature free enzyme investig...

Effects of thermal transitions (glass transition, crystallization), water, and glycerol on invertase activity in freeze-dried carbohydrate systems were investigated. Efficacy of κ-carrageenan to delay sugar crystallization in freeze-dried carbohydrate systems was also studied. Water sorption properties and glass transition temperatures, Tg, were determined for freeze-dried carbohydrate-sucrose ...

The lacto-N-biosidase LnbB from Bifidobacterium bifidum JCM 1254 was engineered to improve its negligible transglycosylation efficiency with the purpose of enzymatically synthesizing lacto-N-tetraose (LNT; Gal-?1,3-GlcNAc-?1,3-Gal-?1,4-Glc) in one enzymatic step. LNT is a prebiotic human milk oligosaccharide itself and constitutes structural core range more complex oligosaccharides as well. Thi...

The sequential changes in intestinal lactase activity of 40 neonates were measured indirectly from the differential uptake and excretion of lactose and the non-metabolisable disaccharide lactulose contained in formula feeds. A daily decline in urinary lactose:lactulose excretion ratios, reflecting a rise in intestinal lactase activity, followed formula feeding. Percentage decline was related di...

Trichosporon cutaneum is shown to utilize six disaccharides, cellobiose, maltose, lactose, sucrose, melibiose, and trehalose. T. cutaneum can thus be counted with the rather restricted group of yeasts (11 to 12% of all investigated) which can utilize lactose and melibiose. The half-saturation constants for uptake were 10 +/- 3 mM sucrose or lactose and 5 +/- 1 mM maltose, which is of the same o...

BACKGROUND Lactase is an enzyme involved in the hydrolysis of lactose. Deficiency of the enzyme (hypolactasia) may be determined genetically or arise secondarily to disease of small intestine. Under this condition, lactose enters the colon where it is fermented by intestinal microflora and turns to gases and short-chain fatty acids, causing gastrointestinal symptoms known as lactose intolerance...

Introduction and Aim: The enzyme beta-galactosidase is extremely important in the food pharmaceutical industries. goal of this work was to improve lactose hydrolysis process employing convert glucose galactose for alleviating intolerance. Methods: Intracellular extracted from Lactiplantibacillus spp. (GV54 GV66) by cell permeabilized method using toluene/acetone solvents. Lactose performed 50 g...