We offer an assessment of the accuracy of the thin-layer agarose fluorescent technique of Elevitch et al. [Am. J. Clin. Pathol. 46, 692 (1966)]. We used semi-purified human lactate dehydrogenase isoenzymes 1 and 5. Both the lactate lead to pyruvate and pyruvate lead to lactate assays [Clin. Chem. 20, 1462 (1974)] appear to give, within the errors of the techniques used, a substantially unbiased...

Optimal reaction conditions to sassay human lactate dehydrogenase (lactate-to-pyruvate) were established for isoenzymes 1 and 5 at 25, 30, and 37 degrees C in diethanolamine and 2-amino-2-methyl-1,3-propanediol. Different substrate concentrations are required at each temperature. The conditions permit measurement of lactate dehydrogenase 1 and 5 with the lowest substrate concentrations that all...

abstract background: athletes’ recovery is important in improving their performance. nutritional strategies can be effective in enhancing recovery rate. choosing the best food items in appropriate intervals can play effective roles in resynthesis of fuels and recovery of muscle injury. beverage micro and macronutrient content are helpful in fuel restoration. in this study, we assess the effects...

The gene encoding D-lactate dehydrogenase (D-lactate: NAD+ oxidoreductase, EC 1.1.1.28) of Lactobacillus plantarum has been sequenced, and expressed in Escherichia coli cells with an inducible expression plasmid, in which the 5‘-noncoding region of the gene was replaced with the tac promoter. Comparison of the sequence of D-laCtate dehydrogenase with L-lactate dehydrogenases, including the L. p...

background: parasite lactate dehydrogenase ( p ldh) is extensively employed as malaria rapid diagnostic tests (rdts). moreover, it is a well-known drug target candidate. however, the genetic diversity of this gene might influence perfor­mance of rdt kits and its drug target candidacy. this study aimed to determine polymorphism of p ldh gene from iranian isolates of p. vivax and p. falciparum. m...

An unusual isozyme of lactate dehydrogenase, láclate dehydrogenase associated with Kirsten murine sarcoma virus (LDHk) was found in the sera of many patients with malignant tumors, while the sera of healthy persons had little or no such activity. This isozyme was detectable only when assayed in a nitrogen atmosphere, and its activity showed little or no relationship to the total lactate dehydr...

This assay for 3-hydroxybutyrate in plasma or serum, based on kinetic initial-rate techniques, has been optimized with respect to initial rate and linearity as follows: pH 8.5, hydroxybutyrate dehydrogenase 62.5 U/L, and NAD+ concentration 20 mmol/L. We have used the assay satisfactorily with both the Gilford 103 and Hitachi 705 discrete analyzers, obtaining results that compare well with those...