To the Editor—Emergence of multidrug-resistant organisms is a threat to public health, because there is limited effort devoted to the development of new antibiotics [1]. Multiple new mechanisms of resistance have been described recently, including a new type of carbapenem resistance gene, blaNDM-1, reported in 2009 [2]. Infections due to strains with blaNDM-1 have been reported in India, Pakist...

This study reports the increasing prevalence of clinical Escherichia coli of sequence type 167 (ST167) carrying both blaNDM-1 and blaNDM-5 on the conjugative IncX3 plasmid in various parts of China. Close surveillance is needed to monitor the future dissemination of ST167 strains that harbor blaNDM-5 or other blaNDM-like genes.

The objective of this study was to reveal the molecular mechanism involved in carbapenem resistance and virulence of a K2 Klebsiella pneumoniae clinical isolate 24835. The virulence of the strain was determined by in vitro and in vivo methods. The de novo whole-genome sequencing technology and molecular biology methods were used to analyze the genomic features associated with the carbapenem res...

To the Editor—The emergence of New Delhi metallo-beta-lactamase–1 (NDM1) in India, Pakistan, and the UK has sparked great fear of the threat posed by resistant microbial strains and by the use of antibiotics worldwide [1]. Thus far, NDM-1 carbapenemase has been detected in several countries in a diverse group of bacteria. Acinetobacter baumannii has emerged as one of the most troublesome pathog...

Antibiotic resistance (AR) is often rooted in inappropriate antibiotic use, but poor water quality and inadequate sanitation exacerbate the problem, especially in emerging countries. An example is increasing multi-AR due to mobile carbapenemases, such as NDM-1 protein (coded by blaNDM-1 genes), which can produce extreme drug-resistant phenotypes. In 2010, NDM-1 positive isolates and blaNDM-1 ge...

blaNDM-1. Diagn Microbiol Infect Dis. 2014; 78:93–7. http://dx.doi.org/10.1016/j.diagmicrobio.2013.08.001 5. Li JJ, Lee CS, Sheng JF, Doi Y. Complete sequence of a conjugative IncN plasmid harboring blaKPC-2, blaSHV-12, and qnrS1 from an Escherichia coli sequence type 648 strain. Antimicrob Agents Chemother. 2014;58:6974–7. http://dx.doi.org/10.1128/ AAC.03632-14 6. Villa L, Garcia-Fernandez A,...

OBJECTIVES This study screened hospital sewage for the presence of blaNDM. METHODS Colonies grown from plates containing meropenem streaked with hospital sewage were screened for blaNDM by PCR. Species identification was performed by sequencing 16S rRNA genes. Clonal relatedness of isolates was determined by Enterobacterial repetitive intergenic consensus sequence-PCR, sequencing recA and PFG...

Global spread of KPC poses to be a serious threat complicating treatment options in hospital settings. The present study investigates the genetic environment of blaKPC-2 among clinical isolates of Pseudomonas aeruginosa from a tertiary referral hospital of India. The study isolates were collected from different wards and clinics of Silchar Medical College and Hospital, India, from 2012-2013. Th...

BACKGROUND Drug-resistant Providencia rettgeri producing metallo-β-lactamase and 16S rRNA methylase has been reported in several countries. We analyzed P. rettgeri clinical isolates with resistance to carbapenems and aminoglycosides in a hospital in Nepal. METHODS Five clinical isolates of multidrug-resistant P. rettgeri were obtained in a hospital in Nepal. Antimicrobial susceptibilities wer...

Việc gia tăng tỷ lệ Klebsiella pneumoniae kháng carbapenem đang trở thành mối lo của toàn thế giới.1 Các chủng do sinh men carbapenemase hoặc kết hợp với ESBL, AmpC. Trong nghiên cứu này, kiểu gen mã hóa được phát hiện bằng kỹ thuật PCR như blaKPC, blaNDM, blaOXA48, blaVIM, blaIMP trong số 187 carbapenemase. Kiểu phổ biến nhất là blaKPC (58,3%), blaNDM (21,4%) và blaOXA-48-like (18,7%). Nghiên ...