Sandßies collectedbetweenApril 2003 andNovember 2004 atTallilAirBase, Iraq,were evaluated for the presence of Leishmania parasites using a combination of a real-time Leishmaniageneric polymerase chain reaction (PCR) assay and sequencing of a 360-bp fragment of the glucose6-phosphate-isomerase (GPI)gene.A total of 2,505pools containing 26,574 sandßieswere testedusing the real-time PCR assay.Leis...

Background Despite the high prevalence and drug resistance of disease in Sistan and Baluchestan Province of Iran, the species of cutaneous leishmaniasis (CL) has not been identified. In the present study, cytochrome b (Cyt b) was used in Sistan and Baluchestan to find species of Leishmania in suspected patients of CL using PCR-RFLP and DNA sequencing. Methods This study was conducted from Oct...

objective(s) leishmania can lead to a broad spectrum of diseases, collectively known as leishmaniasis. the a2 gene/ protein family could be one of the most eligible candidate factors of virulence in visceral leishmaniasis (vl). the previous results confirmed that in leishmania infantum, several a2 proteins are abundantly expressed by the amastigote, but not the promastigote stage. as there are ...

A 5-month old puppy with muco-cutaneous lesions in the chin, around lips and eyes was examined physically and microscopically for leishmaniasis. Muco-cutaneous lesions containing a large number of amastigotes of Leishmania spp. were observed. Amastigotes were also detected in liver and spleen of the puppy. The animal was positive with Dipstick rK39 kit and high level of anti-Leishmania antibodi...

BACKGROUND Cutaneous leishmaniasis (CL) is a vector-borne parasitic disease characterized by the presence of one or more lesions on the skin that usually heal spontaneously after a few months. Most cases of CL worldwide occur in Southwest Asia, Africa and South America, and a number of cases have been reported among troops deployed to Afghanistan. No vaccines are available against this disease,...

We report here the emergence of a new focus of cutaneous leishmaniasis (CL) due to Leishmania tropica (L. tropica) in the Ajmer city of Rajasthan, India, a previously non-endemic area. Between January-February 2006, 13 new indigenously acquired cases of CL were diagnosed among the patients attending the Skin and STD department, JLN Hospital, Ajmer. The diagnosis was based on clinical presentati...

A Western blot method that uses antigens from culture promastigote forms of Leishmania (Viannia) braziliensis, L. (Leishmania) amazonensis, L. (Leishmania) tropica, and a trypanosomatid (strain 268T) isolated from naturally infected tomatoes was evaluated for laboratory diagnosis of American tegumentary leishmaniasis (ATL). Serum samples were obtained from 108 patients with ATL (group I), 23 ch...

conclusions l. major is the main species responsible for cutaneous leishmsniasis in four geographical regions of khuzestan province southwestern of iran and nested pcr can be used for diagnosis and leishmania species identification. results 138 (94.5%) out of 146 cases of four regions were l. major and 8(5.5%) were l. tropica. 57.97% of l. major cases were male and 42.03% were female. 87.5% of ...

background: viscerotropic leishmaniasis caused by leishmania tropica poses a significant prob­lem in the diagnosis and treatment management. since differential gene expression is more im­portant in outcome of the infection, we employed proteomic approach to identify potential pro­teins involved in visceralization of l. tropica . methods: the proteomes profiling of l. tropica isolated from cutan...

In order to identify and characterise the organisms responsible for Cutaneous Leishmaniasis, parasites were isolated from active lesions, grown in-vitro cultures and identified by iso-enzyme characterisation. Thirteen isolates from different patients were typed as L. tropica. Seven of these isolates were from Afghan refugees encamped in the suburbs of Islamabad, 3 were from patients in Multan, ...