This article comprises detail information about Lasparaginase, encompassing topic such as various sources of Lasparaginase, mechanism and properties of L-asparaginase. Also describe the production, cultivation and purification of Lasparaginase along with information about the application of Lasparaginase. L-asparaginase catalyzes the conversion reaction to convert asparagine to aspartic acid an...

Endophytes are novel sources of natural bioactive compounds. This study seeks endophytes that produce the anticancer enzyme l-asparaginase, to harness their potential for mass production. Four plants with anticancer properties; Cymbopogon citratus, Murraya koenigii, Oldenlandia diffusa and Pereskia bleo, were selected as host plants. l-Asparaginase-producing endophytes were detected by the form...

This article comprises detail information about Lasparaginase, encompassing topic such as various sources of Lasparaginase, mechanism and properties of L-asparaginase. Also describe the production, cultivation and purification of Lasparaginase along with information about the application of Lasparaginase. L-asparaginase catalyzes the conversion reaction to convert asparagine to aspartic acid an...

Secondary Diabetes Mellitus (DM) has been associated in about 1-14% of patients with hematologic malignancy treated with L-asparaginase. An 11-year old Filipino female, diagnosed with Acute Lymphoblastic Leukemia, developed hyperglycemia associated with polyuria and polydipsia while on-going induction chemotherapy. There was no family history of diabetes mellitus. Chemotherapy drugs included L-...

Saccharomyces cerevisiae X2180-1A synthesizes two forms of asparaginase: L-asparaginase I, an internal constitutive enzyme, and asparaginase II, an external enzyme which is secreted in response to nitrogen starvation. The two enzymes are biochemically and genetically distinct. The structural gene for asparaginase I (asp 1) is closely linked to the trp 4 gene on chromosome IV. The gene controlli...

In a series of 40 patients treated with L-asparaginase for various neoplastic diseases, 6 patients had generalized anaphylactic reactions to L-asparaginase. Each of these reactors had antibodies detectable by passive hemagglutination, but precipitins were detectable in only one of this group of six patients. That patient had received two courses of the enzyme. 1 wk after the anaphylactic reacti...

L-asparaginase is an enzyme used as a chemotherapeutic agent, mainly for treating acute lymphoblastic leukemia. In this study, the gene of L-asparaginase from Zymomonas mobilis was cloned in pET vectors, fused to a histidine tag, and had its codons optimized. The L-asparaginase was expressed extracellularly and intracellularly (cytoplasmically) in Escherichia coli in far larger quantities than ...

Enzyme L-asparaginase (L-asparagines amidohydrolase EC 3.5.1.1) has the physiological function of hydrolyzing amide group of the side chain in Lasparagine to produce L-aspartate and ammonia. This spectacular property of the enzyme is utilized in chemotherapeutic treatment of certain kinds of lymphoblastic malignancies, mainly in acute lymphoblastic leukaemia (ALL) and lympho-sarcoma for nearly ...

Bacterial L-asparaginases, which are widely used in the antitumor therapy, act only as homotetramers, because their active sites are located at the interface between the subunits of the enzyme. Since salt bridges substantially stabilize L-asparaginase tetramers, we have supposed that oligomerization of bacterial L-asparaginase is a high-avidity process. This assumption was proved by bioinformat...

This study prospectively analyzed the efficacy of very prolonged courses of pegylated Escherichia coli asparaginase (PEGasparaginase) and Erwinia asparaginase in pediatric acute lymphoblastic leukemia (ALL) patients. Patients received 15 PEGasparaginase infusions (2500 IU/m(2) every 2 weeks) in intensification after receiving native E coli asparaginase in induction. In case of allergy to or sil...