Leishmaniasis is a neglected disease with a broad clinical spectrum which includes asymptomatic infection. A thorough diagnosis, able to distinguish and quantify Leishmania parasites in a clinical sample, constitutes a key step in choosing an appropriate therapy, making an accurate prognosis and performing epidemiological studies. Several molecular techniques have been shown to be effective in ...

Maxicircle DNA has been isolated from the kinetoplast DNA of Leishmania tarentolae culture forms by bouyant separation in CsCl in the presence of the A + T-binding dye Hoechst 33258, after liberation from the kinetoplast DNA network by cleavage with the single-hit restriction endonuclease EcoRI. The purified linearized maxicircle DNA has a density in CsCl of 1.681 g/cm3 (79% A + T) and a molecu...

The order Kinetoplastida is composed of several families of flagellated protozoa which are among the most ancient of the eukaryotes. The most prominent members of this order are parasites that are responsible for major tropical diseases. These parasites include Trypanosoma brucei, the African trypanosome; Trypanosoma cruzi, the South American trypanosome; and several species of Leishmania. Othe...

objective: ajowan is an annual herbaceous essential oil of carum copticom. the main components of the oil are tymol, β-pinene, γ- terpinene and sabinene.the fruit oil of carum copticum has been reported to have several therapeutic effects including anti fungal, anti bacterial and anti viral,... candida albicans is an opportunistic fungus and transforms into pathogenic form in favorable conditio...

We discuss here some results which suggest that radically different maxicircle classes coexist within the same kinetoplast. These data, although tentative and incomplete, may provide a new outlook on the kinetoplast genome structure and expression. Report The kinetoplast genome is composed of a large heterogeneous population of minicircles and a small number of maxicircles. The maxicircles cont...