BACKGROUND Epidemiologic evidence on the association of individual antioxidant vitamins and cognition is inconsistent. OBJECTIVE We evaluated the total antioxidant capacity of diets on the basis of the ferric-reducing antioxidant power (FRAP) assay in relation to cognition in older women. DESIGN Starting in 1995, we used a telephone-based cognitive assessment to evaluate cognitive function ...

Objective(s): Gentamicin is used for the treatment of Gram-negative bacterial infections. However, gentamicin administration is limited because of nephrotoxicity. The aim of the present study was to evaluate the protective effect of crocin against gentamicin-induced nephrotoxicity in rats. Materials and Methods: Thirty two male Wistar rats received gentamicin (100 mg/kg, IP), with or without c...

BACKGROUND Macromolecule mobility is often quantified with Fluorescence Recovery After Photobleaching (FRAP). Throughout literature a wide range of diffusion coefficients for GFP in the cytoplasm of Escherichia coli (3 to 14 µm²/s) is reported using FRAP-based approaches. In this study, we have evaluated two of these methods: pulsed-FRAP and "conventional"-FRAP. PRINCIPAL FINDINGS To address ...

PURPOSE The comparison of nutrition of children with high level of physical activity in dependence on antioxidative efficiency expressed in Ferric Reducing Ability of Plasma--FRAP adapted for saliva. MATERIAL AND METHODS The group consisted of 74 pupils (43 boys and 31 girls) from swimming classes of Sport Championship School in Kraków. FRAP was measured in saliva with the use of colorimetric...

objective: boerhaavia elegans l. (nyctaginaceae) is a medicinal plant used for the treatment of kidney disorders, urinary tract disorders and blood purification in baluch tribe. the aim of present study is to evaluate the antioxidant propertyof b. elegans species for the first time. materials and methods: different parts (leaf, stem and fruit) of the plant were extracted by using various solven...

objective(s): gentamicin is used for the treatment of gram-negative bacterial infections. however, gentamicin administration is limited because of nephrotoxicity. the aim of the present study was to evaluate the protective effect of crocin against gentamicin-induced nephrotoxicity in rats. materials and methods: thirty two male wistar rats received gentamicin (100 mg/kg, ip), with or without cr...

The FRAP-p70 signaling pathway was found to be constitutively phosphorylated/active in MiaPaCa-2 and Panc-1 human pancreatic cancer cells and a pancreatic cancer tissue sample as judged by the retarded electrophoretic mobility of the two major FRAP downstream targets, p70 and 4E-BP1. Treatment of cells with rapamycin, a selective FRAP inhibitor, inhibited basal p70 kinase activity and induced d...

Confocal fluorescence recovery after photobleaching (FRAP) is today the prevalent tool when studying the diffusional and kinetic properties of proteins in living cells. Obtaining quantitative data for diffusion coefficients via FRAP, however, is challenged by the fact that both bleaching and scanning take a finite time. Starting from an experimental case, it is shown by means of computer simula...

The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5' end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5' cap structure. Assembly of the eIF4F complex is inhibited by a family of repressor polypeptides, the eIF4E-binding proteins (4E-BPs). Binding of the 4E-BPs to eIF4E is regulated by phosphorylation: Hypophosphorylate...