Lyme disease caused by Borrelia burgdorferi is the most common tick-borne disease in the US and Europe. Unlike most bacteria, measurements of growth and viability of B. burgdorferi are challenging. The current B. burgdorferi viability assays based on microscopic counting and PCR are cumbersome and tedious and cannot be used in a high throughput format. Here, we evaluated several commonly used v...

Various methods for the recovery and detection of HAV have been suggested, and molecular tests have recently provided an effective replacement for the traditional methods. Real-time RT-PCR technology offers many advantages over conventional RT-PCR in terms of rapidity and specificity. Most procedures are based on the TaqMan chemistry, but some researchers have used the SYBR Green I approach, wh...

A simple, non-destructive procedure is described to determine the quality of DNA arrays before they are used. It consists of a preliminary staining step of the DNA microarray by using SYBR green II, a fluorophore with specific affinity for ssDNA, followed by a laser scan analysis. The surface quality, integrity and homogeneity of each DNA spot of the array can thus be assessed. After this preli...

Barmah Forest virus (BFV) is a mosquito borne (+) ssRNA alphavirus found only in Australia. It causes rash, myalgia and arthralgia in humans and is usually diagnosed serologically. We developed a real-time PCR assay to detect BFV in an effort to improve diagnosis early in the course of infection. The limit of detection was 16 genome equivalents with a specificity of 100%. Fifty five serum sampl...

conclusions multiplex sybr green real-time pcr is a rapid and relatively inexpensive method for detection of respiratory viruses. results application of the multiplex sybr green real-time pcr in clinical samples from 172 patients in a one-year study resulted in detection of 19 (11.04%) piv3, 9 (5.23%) piv4, and 1 (0.58%) coronavirus nl63. all the positive samples were detected during december t...

Flow cytometry can rapidly quantitate bacteria and discriminate them from debris. Researchers at the Swiss Federal Institute of Aquatic Science and Technology (Eawag, Switzerland) have developed a standard flow cytometric staining protocol and a corresponding BD AccuriTM C6 software analysis template to discriminate bacteria from debris in drinking water samples. The template employs a single, ...

background: nowadays, scourge of malaria as a fatalistic disease has increased due to emergence of drug resistance and tolerance among different strains of plasmodium falciparum . emergence of chloroquine (cq) resistance has worsened the calamity as cq is still considered the most efficient, safe and cost effective drug among other antimalarials. this urged the scientists to search for other al...

The expression gene pattern reflects, in part, mechanisms involved in adaptation to environmental conditions. Thus, we established and validated a method that enables relative transcript quantification in different conditions in the lactic acid bacteria Oenococcus oeni, notably in a technological medium. First, we determined an internal control in our conditions by reverse transcription quantit...

Duck spleen necrosis disease (DSND) caused by Novel Reovirus (NDRV), is an emerging infectious that causes severely threaten to duck industry. Currently, the popular conventional RT-PCR technique for detecting NDRV time consuming. So, it essential develop a rapid and accurate molecular diagnosis techniques of pathogen purpose effective control disease. In our study, simple, reliable detection m...