The binding of bovine testicular hyaluronidase to AH-Sepharose (1,6-diaminohexane--Sepharose) gels substituted with (1) dermatan sulphate, (2) desulphated dermatan sulphate, (3) heparin and (4) de-N/O-sulphated, re-N-acetylated heparin was investigated. Hyaluronidase was found to bind to (1) and (3), but not (2) and (4). On the basis of these observations a preparative scheme for the purificati...

I t is generally agreed that there is a close relationship between spreading factors (S. F.) from testis, bacteria, venoms, and other sources with the mucolyric enzyme, hyaluronidase (cf. the review of Duran-Reynals (1) for references). While it is known that hyaluronidase removes a dermal barrier (presumably hyaluronic acid) to the diffusion of fluids in skin certain aspects of hyaluronidase s...

1. Human synovial fluid was analysed electrophoretically before and after treatment with testicular hyaluronidase. 2. After hyaluronidase treatment, the electrophoretic mobilities of the synovial proteins decreased markedly and were similar to the values obtained for blood serum proteins. 3. The 1r-component, a complex of hyaluronic acid and albumin, could not be detected after hyaluronidase tr...

A double-blind trial demonstrates the effectiveness of adding hyaluronidase to lignocaine with adrenaline in producing ocular akinesia and anaesthesia in retrobulbar nerve blocks. 92% of the blocks in which hyaluronidase was used for intracapsular cataract surgery were judged successful compared with 56% of those without added hyaluronidase (p less than 0.01).

Hyaluronidase has been shown to increase the rate of spread of intradermally injected solutions in animals (Chain and Duthie, 1939, 1940) and the whole subject of spreading factors was reviewed by Duran-Reynals (1942). Holborow and Keech (1951) studied the effects of hyaluronidase in accelerating the rate of spread of a solution of haemoglobin injected intradermally in normal human subjects and...

To test the hypothesis that epidural administration of lidocaine, xylazine or xylazine plus hyaluronidase provides reduced pain and stress during electroejaculation in bulls, eight 30-month-old Nellore bulls received saline solution (control), 2% lidocaine, 2% xylazine or 2% xylazine plus hyaluronidase injected into the first intercoccygeal (Co1-Co2) epidural space in randomized order. Heart ra...

OBJECTIVE To evaluate the efficacy of hyaluronidase in preventing increases in intraocular pressure related to injections of hyaluronan-containing viscoelastic substances. METHODS Twenty-five white rabbits were divided into 5 groups. In groups 1 through 4, 0.15 mL of aqueous humor was removed and replaced with 0.10 mL of a viscoelastic substance in both eyes. Additionally, 10 units of hyaluro...

Hyaluronidases degrade hyaluronic acid, a major polysaccharide of the extracellular matrix of tissues, and are considered important for virulence in a number of Gram-positive and -negative bacteria. The purpose of the present study was to determine the prevalence of hyaluronidase among clinical strains of Staphylococcus aureus and among other Staphylococcus species. Spent media and chromosomal ...

It has been shown that intravenously injected bovine testicular hyaluronidase reduces ischemic myocardial necrosis in animal models of myocardial infarction. The mechanism for the protective effect of hyaluronidase is unknown, but it has been suggested that this enzyme depolymerizes myocardial hyaluronic acid and thus improves interstitial transport. However, biochemical data showing that intra...