Seven species of Microsporum, 4 of Trichophyton and 1 Epidermophyton including 55 species belonging to 33 genera were studied for extracellular keratinase. The preliminary screening was done as their ability to develop clearing zone formation. The keratinase of Microsporum species ranged from 50-300ku/ml and of Trichophyton 100-140ku/ml while that of Epidermophyton was 20ku/ml.

background one group of significant enzymes produced by bacillus genus are alkaline proteases with several important applications in the daily life as well as common industries such as food, detergents, leather, alcohol and beer production and medical and sanitary industries, beside wastewater treatment, , biotransformation, hydrolyzed proteins and oil manufacturing. objectives in this paper, k...

Abstract Incubation parameters used for the creation of a protein lysate from enzymatically degraded waste feathers using crude keratinase produced by Laceyella sacchari strain YNDH were optimized Response Surface Methodology (RSM); amino acids quantification was also estimated. The optimization elevated total to 2089.5 µg/ml through application following optimal conditions: time 20.2 h, feathe...

A novel feather-degrading Bacillus Sp. was isolated from chick manure. The bacterium produces keratinolytic enzyme using chicken feather as a sole of carbon and nitrogen source. The effect of temperature, pH on keratinase production of this keratinolytic strain was studied. Maximum enzyme activities were obtained within 48 hrs (780U/ml) of cultivation. The optimal conditions for the keratinolyt...

Three Bacillus species (B. subtilis LFB-FIOCRUZ 1270, B. subtilis LFB-FIOCRUZ 1273, and B. licheniformis LFB-FIOCRUZ 1274), isolated from the poultry industry, were evaluated for keratinase production using feathers or feather meal as the sole carbon and nitrogen sources in a submerged fermentation. The three Bacillus spp. produced extracellular keratinases and peptidases after 7 days. Feather ...

Based on previous screening for keratinolytic nonpathogenic fungi, Paecilomyces marquandii and Doratomyces microsporus were selected for production of potent keratinases. The enzymes were purified and their main biochemical characteristics were determined (molecular masses, optimal temperature and pH for keratinolytic activity, N-terminal amino acid sequences). Studies of substrate specificity ...

In black feathers, melanin is embedded in keratin matrix that makes feather more resistance to the microbial degradation. Chryseobacterium sp. RBT previously isolated from the poultry waste disposable site revealed strong sulfitolytic and keratinolytic activities. Maximum keratinase activity was observed at 48 h (89.12 U ml-1) showed 83 % of native black feather degradation. The concentration o...

Keratinases are enzymes of great importance involved in pathogenic processes of some fungi. They also have a widespread ecological role since they are responsible for the degradation and recycling of keratin. On the one hand, studying them furthers our knowledge of pathogenicity mechanisms, which has important implications for human health, and on the other hand, understanding their ecological ...