Both protein kinase C (PKC) and adenosine receptor activation have been shown to enhance ATP-sensitive K+(KATP) channels. The present studies were designed to determine whether PKC mediates adenosine effects on the KATP channel. The dependence of KATP channel activity ( nP o) on intracellular ATP concentration ([ATP]i) was determined in excised rabbit ventricular membrane patches. External aden...

BACKGROUND Potassium channels play critical roles in the regulation of cell membrane potential, which is central to the excitability of myometrium. The ATP-sensitive potassium (KATP) channel is one of the most abundant potassium channels in myometrium. The objectives of this study were to investigate the protein expression of KATP channel in human myometrium and determine the levels of KATP cha...

a b s t r a c t introduction: even today there is no effective drug therapy to prevent neuronal loss after brain stroke. in the present study we studied the effect of mitochondrial katp channel regulators on neuronal ultrastructure after ischemia reperfusion in the rat. materials & methods: rats temporarily subjected to four vessels occlusion for 15 minutes followed by 24 hours reperfusion with...

The activation of ATP-sensitive potassium (KATP) channels in PC12 cells play a pivotal role in protection against the neurotoxic effect of rotenone. However, it remains unclear why rotenone seems to preferentially affect activation of KATP channels and if this could affect its physiological activity. In this study, we sought to determine how the d...

Zingman LV, Alekseev AE, Hodgson-Zingman DM, Terzic A. ATP-sensitive potassium channels: metabolic sensing and cardioprotection. J Appl Physiol 103: 1888–1893, 2007. doi:10.1152/japplphysiol.00747.2007.—The cardiovascular system operates under a wide scale of demands, ranging from conditions of rest to extreme stress. How the heart muscle matches rates of ATP production with utilization is an a...

In the present study, we have investigated the mechanism underlying the activation by 5-amino-N-[2-(2-chlorophenyl)ethyl]-N'-cyano-3- pyridinecarboxamidine (KRN4884), a new K+ channel opener, of ATP-sensitive K+ (KATP) channels in single ventricular cells of guinea pig hearts by the inside-out patch-clamp method. In the presence of intracellular ATP (1 mM), KRN4884 (0.1-3 microM) activated KATP...

Objective: To assess the significance of protein kinase A (PKA) in glucose triggering of ATP-sensitive Kþ (KATP) channel-dependent insulin secretion and in glucose amplification of K þ ATP channel-independent insulin secretion. Methods: Insulin release from cultured perifused mouse pancreatic islets was determined by radioimmunoassay. Results: In islets cultured at 5.5 mmol/l glucose, and then ...

Ashcroft FM. ATP-sensitive K channels and disease: from molecule to malady. Am J Physiol Endocrinol Metab 293: E880–E889, 2007. First published July 24, 2007; doi:10.1152/ajpendo.00348.2007.—This essay is based on a lecture given to the American Physiological Society in honor of Walter B. Cannon, an advocate of homeostasis. It focuses on the role of the ATP-sensitive potassium K (KATP) channel ...

Current technologies for studying ion channels are fundamentally limited because of their inability to functionally link ion channel activity to cellular pathways. Herein, we report the use of label-free cell phenotypic profiling to decode the composition and signaling of an endogenous ATP-sensitive potassium ion channel (KATP) in HepG2C3A, a hepatocellular carcinoma cell line. Label-free cell ...

Metabolic-sensing ATP-sensitive K+ channels (KATP channels) adjust membrane excitability to match cellular energetic demand. In the heart, KATP channel activity has been linked to homeostatic shortening of the action potential under stress, yet the requirement of channel function in securing cardiac electrical stability is only partially understood. Here, upon catecholamine challenge, disruptio...