The present study describes the isolation of a protein from Escherichia coli possessing kynurenine aminotransferase (KAT) activity and its identification as aspartate aminotransferase (AspAT). KAT catalyses the transamination of kynurenine and 3-hydroxykynurenine to kynurenic acid and xanthurenic acid respectively, and the enzyme activity can be easily detected in E. coli cells. Separation of t...

The finding that chromatin modifications are sensitive to changes in cellular cofactor levels potentially links altered tumor cell metabolism and gene expression. However, the specific enzymes and metabolites that connect these two processes remain obscure. Characterizing these metabolic-epigenetic axes is critical to understanding how metabolism supports signaling in cancer, and developing the...

Kynurenic acid (KYNA), the only known endogenous glutamate antagonist, is produced in the brain by kynurenine aminotransferases (KATs) I and II. Mitochondrial toxins, 1-methyl-4-phenylpyridinium (MPP +) and 3-nitropropionic acid (3-NPA), were previously shown to reduce KYNA synthesis via interference with KAT I and II. Data presented here demonstrate that immunophilin ligand, FK506 (10-130 micr...

Abstract The cylindrical graphite cavity chambers are used for primary standard of air kerma rate of Co-60 and Cs-137. We measured wall attenuation correction factor (Kat) at several irradiation angles using extrapolation technique because of angular dependence. From these results, it can be seen that it is insufficient technique for evaluation of Kat. The Monte Carlo simulations were also done...

Implication rules have been used in uncertainty reasoning systems to confirm and draw hypotheses or conclusions. However a m_jor bottleneck in developing such systems lies in the elicitation of these rules. This paper empirically examines the performance of evidential inferencing with implication networks generated using a rule induction tool called KAT. KAT utilizes an algorithm for the statis...

JUNO’s investigation focuses on four themes: origin, interior structure, atmospheric composition and dynamics, polar magnetosphere. Using a spinning solar-powered spacecraft, the Juno equipment will obtain global maps of the gravity, magnetic fields, and atmospheric composition of Jupiter from a unique polar orbit with a close perijove. JUNO carries in fact precise, high-sensitivity radiometers...

BACKGROUND Kidney autotransplantation (KAT) is the ultimate approach for nephron-sparing surgery. It is a rarely used method in renal tumor surgery today as minimal invasive and open techniques for nephron-sparing surgery improve constantly. In this publication, the complication rate and the long-term functional and oncological outcome at a single center are analyzed. METHODS A prospectively ...

Planning a path for a nonholonomic robot is a challenging topic in motion planning and it becomes more difficult when the desired path contains narrow passages. This kind of scenario can arise, for instance, when quadcopters search a collapsed building after a natural disaster. Choosing the quadcopter as the target platform, this paper proposes the Kinodynamic Aggressive Trajectory (KAT) motion...

The p300, CBP, and pCAF lysine acetyltransferase (KAT) proteins have been reported to physically interact with bovine (BPV) and human (HPV) papillomavirus E2 proteins. While overexpression of these KAT proteins enhances E2-dependent transcription, the mechanism has not been determined. Using RNA interference (RNAi) to deplete these factors, we demonstrated that E2 transcriptional activity requi...

Prostaglandin-endoperoxide H synthase (PGHS) (EC 1.14.99.1) expression was examined in human thyroid tissue and in KAT-50, a well differentiated human thyroid epithelial cell line. PGHS-1 is found constitutively expressed in most healthy tissues, whereas PGHS-2 is highly inducible and currently thought to be expressed, with few exceptions, only in diseased tissues. Surprisingly, PGHS-2 mRNA and...