background traditional medicines are among the oldest medicines and their extensive use in the recent years reflects the public’s interest in alternatives to conventional medicine. objectives the aim of this study was to investigate the genotoxicity of dillsun herbal medicine in dna damage of rat hepatocytes compared to sodium dichromate using a comet assay technique. materials and methods male...

background: differentiation ofmesenchymal stem cells (mscs) to hepatocyte-like cells could be associated with development of liver function factors. the impact of differentiation-dependent changes on dna integrity is not well understood. in this study, hepatocytes and their progenitor stem cells were treated with aflatoxin b1 (afb1) and amplification of selected genes linked to dna damage was e...

objective(s): stem cell therapy is believed to be as a promising treatment strategy for tissue repair and regeneration. the plasticity specification of the adult stem cells, such as mscs, has enabled that these cells to be used in the treatment of a broad spectrum of diseases like liver disorders. in this study, the production of urea and albumin (alb), glycogen storage, and expression of some ...

cobalt is an essential co-factor in red blood cell production and function and its deficiency may produce clinical signs in sheep. thus, present study was designed to evaluate the effect of cobalt nano-particles on serum biochemical factors and histopathological changes in liver and kidnies of lambs. study was carried out in 3 groups of lambs (4 lambs per group). one group of lambs was kept as ...

results at high concentrations (40 and 100 μm), apoptosis was demonstrated and it was clearly more prominent in hepatocytes exposed to celecoxib loaded liposomes. conclusions our results showed that celecoxib loaded liposomes robustly induced apoptosis, an action that can potentially make it more relevant than the usual form for chemopreventive strategies and also may raise concerns about its t...

background the increasing use of herbal drugs and their ease of accessibility and availability have necessitated the use of mutagenicity test to analyze their toxicity and safety. objectives this study aimed to evaluate the genotoxicity of sankol herbal medicine in dna breakage of rat hepatocytes in comparison with h2o2 by single cell gel electrophoresis technique or comet assay. materials and ...

conclusions hepatocyte isolation can be performed as the main and initial step for cell transplantation from unused/rejected liver. it is the first experience in iran. results highly quality hepatocytes with high viability and low contamination were isolated. the mean viability was 71.8% ± 21.7. in the first 4 cases microbial contamination by staphylococci, diphtheroid and klebsiella was detect...

Thirty-six cases of necrotizing lymphadenitis--including 33 cases of unknown etiology, 1 typhoid lymphadenopathy, and 2 cases of suspicious lupus lymphadenopathy--were clinico-pathologically reviewed and analyzed with immunostaining for s-100 and lysozyme. All cases histologically showed architectural effacement by paracortical lesions composed of nuclear karyorrhexis and mononuclear cell proli...

T-2 toxin (3 mg/kg b.w.) was orally inoculated to pregnant mice at 11 days of gestation to examine the effect of T-2 toxin on the developing embryos. At 24 hours after T-2 toxin-inoculation, moderate pyknosis or karyorrhexis was generally observed in some layers of the central nervous system, caudal sclerotomic segment, caudal region of the tongue to pharyngeal- to laryngeal-mesenchyma, trachea...