Two Leuconostoc mesenteroides strains NCDC 744 and NCDC 745 were evaluated for exopolysaccharides (EPS) production in whey and MRS medium. Paneer whey medium supplemented with 10% sucrose, 0.1 % yeast extract and 0.1 % K2HPO4 was found to be proferred medium for EPS production. Among two strains L. mesenteroides NCDC 744 produced significantly higher EPS (12.7 ± 0.24 gm/L) as compared to NCDC 7...

Mouse liver mitochondria were prepared as described and resuspended in MRM buffer (250 mM sucrose, 10 mM HEPES, pH 7.5, 1 mM ATP, 5 mM sodium succinate, 80 mM ADP, 2 mM K2HPO4) at a concentration of 0.5 mg ml 21 (ref. 18). Rat liver mitochondria were isolated from 4–6-month-old Fischer 344 £ brown Norway F1 rats by differential centrifugation as described, except the final wash and resuspension...

A chemically defined medium was devised in order to study the requirements for fortimicin A production by Micromonospora olivoasterospora KY 11515. Soluble starch was the best carbon source; NH4Cl and NH4NO3 were suitable nitrogen sources both for the growth and fortimicin production. Amino acids such as L-asparagine, L-aspartic acid and L-glutamic acid showed some stimulatory effects on both g...

A simple digital image processing technique is examined to recognize the phase separation of a chemical process. traditional aqueous-organic hexane (1) - water (2) acetic acid (3) system and its alternative aqueous two-phase PEG (1)-K2HPO4 (2)-water was selected as model with or without polymer. In this technique, monitored recorded using webcam set up processed on computer an subtraction proce...

The global use of pesticides has resulted in the contamination of various ecosystems worldwide. The impact of these pesticides can be reduced through bioremediation. The factors that influence the biodegradation rate include the isolation of efficient bacteria for use in remediation and the determination of optimal biodegradation conditions. In this study, malathion degrading bacteria were isol...

Weare reporting an efficient method for microbial production of 4-thiouridine as an alternative to chemical synthesis. A loopful of a 10-day slant culture of S. libani NK-1 10134 grown on yeast extractstarch agar was inoculated into a 500-ml Erlenmeyer flask containing 1 10 ml ofa seed mediumcomposed of soluble starch 2.0%, glucose 0.5%, peptone 0.5%, yeast extract 0.5%, soybeanmeal0.5%, K2HPO4...

search for novel antimicrobial agents from microorganisms, an actinomycete strain Streptomyces sp. isolated from a soil sample collected in Taejon, Korea, was found to produce a new antibacterial 16-methyloxazolomycin (1). In this paper, we describe the production, isolation, structural elucidation and biological activities of 1. The strain was cultured in the seed mediumconsisting of glucose 2...