نتایج جستجو برای: its4

تعداد نتایج: 316  

Journal: :The Indian journal of medical research 2006
Bansidhar Tarai A Gupta P Ray M R Shivaprakash A Chakrabarti

BACKGROUND AND OBJECTIVES Fungal endophthalmitis is a devastating form of ocular infection and postoperative endophthalmitis following cataract surgery is the commonest form of such infection. Early diagnosis is very important for effective management of these patients. As conventional techniques take longer time and lacks sensitivity, polymerase chain reaction (PCR) for detection of fungal DNA...

Journal: :Genetics and molecular research : GMR 2012
M A Hameed R M Rana Z Ali

Crown rot is one of the main important fungal diseases affecting wheat in many areas of the world, including Australia, USA, and Iran. Until now, there had been no report of this pathogen in Iraq. Plants displaying crown rot symptoms were observed in Shaat Alarab (Basra, Iraq); we investigated the causal agent of the disease. Samples were surface-sterilized in bleach (1% available chlorine...

2014
Kalaivani Nadarajah Nurfarahana Syuhada Omar Marhamah Md Rosli Ong Shin Tze

Two field isolates of Rhizoctonia solani were isolated from infected paddy plants in Malaysia. These isolates were verified via ITS-rDNA analysis that yielded ~720 bp products of the ITS1-5.8S-ITS4 region, respectively. The sequenced products showed insertion and substitution incidences which may result in strain diversity and possible variation in disease severity. These strains showed some re...

2007
J.D.W. Dearnaley A. F. Le Brocque

We have identified the primary root fungal endophytes of Dipodium hamiltonianum F.M. Bailey, a species of orchid endangered in Victoria and uncommon in New South Wales and Queensland. Genomic DNA was extracted from whole colonised root portions from four orchid individuals and PCR amplified with ITS1F and ITS4 primers. Cloning and sequencing of the main amplicons produced from the PCR analysis ...

Journal: : 2022

Hoạt động gây trồng hoa lan đang phát triển mạnh ở Việt Nam, đặc biệt là Phi điệp. Tại Thái Nguyên, các vườn thường xuyên xuất hiện bệnh loét thân trên cây Nghiên cứu này nhằm mô tả điểm hình thái, định loại và tính của chủng nấm thuộc chi Fusarium phân lập từ bị tại Nguyên. Triệu chứng điển có vết loét, lá chuyển vàng, héo, khô chết. Kết quả giám bằng phương pháp chỉ thị tử với việc sử dụng cặ...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه لرستان - دانشکده کشاورزی 1394

قارچ فوزاریوم یکی از جنس های قارچی مهم بیماری زای گیاهی می باشد که گونه های آن هر ساله خسارت زیادی را به محصولات کشاورزی وارد می کنند. استفاده از ارقام مقاوم یکی از موثرترین روش-های کنترل بیماری های ناشی از این قارچ ها به حساب می آید، اما به منظور انتخاب و توسعه ارقام مقاوم، آگاهی از تنوع ژنتیکی جمعیت های این قارچ ها ضروری است. در این مطالعه 77 جدایه از فوزاریوم ها از نظر مورفولوژیکی مورد بررسی...

Journal: :Jurnal Fitopatologi 2023

Morphological characterization of Phytophthora isolates commonly was done on the basis colony and hyphal swelling characteristics, form, shape chlamydospore, sporangia as well oogonia antheridia. Molecular conducted using specific primer pairs Pal1 s Pal2a for palmivora universal ITS4 ITS5 followed by sequencing isolates. The virulence tested a cocoa pod Sulawesi2 clone. A total 21 were collect...

Journal: :Egyptian Academic Journal of Biological Sciences, H. Botany 2021

Ficus species (Moraceae) is a globally distributed and considered one of the largest most diverse genera woody plants with growing genetic, environmental, economic, nutritional, medicinal importance. This study was conducted to detect genetic evolutionary relationships among eight genus (Ficus microcarpa var nitida, F. retusa, benjamina Vivian, Starlight, cyathistipula, elastica Decora binnendi...

ژورنال: :رستنیها 2006
سعیده دهقانپور فراشاه بهرام شریف نبی آقا فخر میرلوحی

در این پژوهش قارچ های اندوفیت از غلاف برگ و بذرها میزبان های گیاهیf. arundinacea، f. pratensis، festuca ovina، bromus tomentellus، melica persica و lolium prenne جداسازی گردید. پس از استخراج dna ژنومی این جدایه ها به منظور تایید حضور گونه های اندوفیت از سه جفت آغازگر its1/its4، is1/is3 و 111/112 استفاده گردید. نتایج حاصل از pcr با این سه جفت آغازگر نشان داد که اکثر جدایه های مورد استفاده در این...

Journal: :iranian journal of public health 0
h mirhendi a ghiasian hf vismer mr asgary n jalalizand mc arendrup

background: fusarium species are capable of causing a wide range of crop plants infections as well as uncommon hu­man infections. many species of the genus produce mycotoxins, which are responsible for acute or chronic diseases in ani­mals and humans. identification of fusaria to the species level is necessary for biological, epidemiological, pathologi­cal, and toxicological purposes. in this s...

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