نتایج جستجو برای: itd mutation

تعداد نتایج: 292736  

Journal: :Croatian medical journal 2008
Hong-Ling Peng Guang-Sen Zhang Fan-Jie Gong Jian-Kai Shen Yang Zhang Yun-Xiao Xu Wen-Li Zheng Chong-Wen Dai Min-Fei Pei Jun-Jie Yang

AIM To assess the expression level of fms-like tyrosine kinase 3 (FLT3), the incidence of FLT3/internal tandem duplications (ITD) mutation, and prognostic value of FLT3 changes in different types of adult leukemia. METHODS Bone marrow mononuclear cells were isolated from 147 adult patients with leukemia. Reverse transcriptase polymerase chain reaction (PCR) was used to screen FLT3/ITD mutatio...

2016
CHENGLONG LI BIAO ZHU JIAO CHEN XIAOBING HUANG

In the present study, gene expression profiles of acute myeloid leukemia (AML) samples were analyzed to identify feature genes with the capacity to predict the mutation status of FLT3/ITD. Two machine learning models, namely the support vector machine (SVM) and random forest (RF) methods, were used for classification. Four datasets were downloaded from the European Bioinformatics Institute, two...

2016
Aoli Wang Hong Wu Cheng Chen Chen Hu Ziping Qi Wenchao Wang Kailin Yu Xiaochuan Liu Fengming Zou Zheng Zhao Jiaxin Wu Juan Liu Feiyang Liu Li Wang Richard M. Stone Ilene A. Galinksy James D. Griffin Shanchun Zhang Ellen L. Weisberg Jing Liu Qingsong Liu

The FLT3-ITD mutation is one of the most prevalent oncogenic mutations in AML. Several FLT3 kinase inhibitors have shown impressive activity in clinical evaluation, however clinical responses are usually transient and clinical effects are rapidly lost due to drug resistance. One of the resistance mechanisms in the AML refractory patients involves FLT3-ligand induced reactivation of AKT and/or E...

2015
Y Kim G D Lee J Park J-H Yoon H-J Kim W-S Min M Kim

Mutation of the fms-like tyrosine kinase 3-internal tandem duplication (FLT3-ITD), which is one of the most frequent genetic alterations, strongly contributes to an increased risk of treatment failure and to poor prognosis. In this study, we established quantitative fragment analysis of FLT3-ITD simultaneously measuring mutant allele burden and length, verified the analytical performance and ev...

2011
Sawami Suzuki Hiroko Inaba Takashi Satoh Toshio Okazaki Shinichiro Takahashi

Flt3-internal tandem duplications (Flt3-ITD) is a prevalent mutation in acute myeloid leukemia (AML). We recently reported arsenic trioxide (ATO) and Flt3 inhibition synergize to induce apoptosis in Flt3-ITD cells. However, the signaling effect of ATO in these cells has not been elucidated. Here, we demonstrate that the treatment of ATO potently induces the activation of extracellular regulated...

Journal: :Blood 2008
Li Li Obdulio Piloto Ho Bao Nguyen Kathleen Greenberg Kogo Takamiya Frederick Racke David Huso Donald Small

Constitutive activation of FMS-like tyrosine kinase 3 (FLT3) by internal tandem duplication (ITD) mutations is one of the most common molecular alterations known in acute myeloid leukemia (AML). To investigate the role FLT3/ITD mutations play in the development of leukemia, we generated a FLT3/ITD knock-in mouse model by inserting an ITD mutation into the juxtamembrane domain of murine Flt3. FL...

ژورنال: :فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران 0
مریم شیخی maryam sheikhi msc, department of genetics, science and research campus, islamic azad university, tehran, iran. فرهاد ذاکر farhad zaker associate professor, department of hematology, iran university of medical sciences, tehran, iran. غلامرضا جوادی gholam reza javadi associate professor, department of genetics, science and research campus, islamic azad university, tehran, iran. مهرداد هاشمی mehrdad hashemi assistant professor, department of genetics, tehran medical branch, islamic azad university, tehran, iran. فرناز رزم خواه farnaz razmkhah msc, department of genetics, iran university of medical sciences, tehran, iran.

سابقه و هدف: جهش ژن flt3 (گیرنده تیروزین کینازی شبه) fms باعث تکثیر بدون کنترل سلول های لوسمیک شده و پیش آگهی بدی را به همراه دارد. هدف ما از این مطالعه اجرایی کردن آزمایشات مولکولی برای تشخیص و غربالگری این جهش ها در مبتلایان لوسمی های حاد بود.روش بررسی: در این مطالعه بنیادی، 91 کودک مبتلا به لوسمی های حاد میلوئیدی (aml) و لنفوئیدی (all) از نظر جهش در ژن flt3 شامل وجود جهش itd (internal tandem...

Journal: :Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2010
Claire L Green Kenneth K Koo Robert K Hills Alan K Burnett David C Linch Rosemary E Gale

PURPOSE To determine the clinical relevance of mutations in the CCAAT/enhancer binding protein alpha (CEBPA) gene in acute myeloid leukemia (AML) and to examine factors that might modify prognostic impact. PATIENTS AND METHODS The entire CEBPA coding sequence was screened in 1,427 young adult patients with AML, excluding acute promyelocytic leukemia, using denaturing high-performance liquid c...

2017
Xia Li Xiufeng Yin Huafeng Wang Jiansong Huang Mengxia Yu Zhixin Ma Chenying Li Yile Zhou Xiao Yan ShuJuan Huang Jie Jin

Acute myeloid leukemia (AML) is a highly heterogeneous disease and internal tandem duplication mutation in FMS-like tyrosine-kinase-3 (FLT3-ITD) has a negative impact on outcome. Finding effective treatment regimens is desperately needed. In this study, we explored the inhibitory effect and mechanism of homoharringtonine (HHT) in combination with ibrutinib on FLT3-ITD mutant AML cells. Conseque...

Journal: :Blood 2012
Nicholas A Zorko Kelsie M Bernot Susan P Whitman Ronald F Siebenaler Elshafa H Ahmed Gabriele G Marcucci Daniel A Yanes Kathleen K McConnell Charlene Mao Chidimma Kalu Xiaoli Zhang David Jarjoura Adrienne M Dorrance Nyla A Heerema Benjamin H Lee Gang Huang Guido Marcucci Michael A Caligiuri

The MLL-partial tandem duplication (PTD) associates with high-risk cytogenetically normal acute myeloid leukemia (AML). Concurrent presence of FLT3-internal tandem duplication (ITD) is observed in 25% of patients with MLL-PTD AML. However, mice expressing either Mll-PTD or Flt3-ITD do not develop AML, suggesting that 2 mutations are necessary for the AML phenotype. Thus, we generated a mouse ex...

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