the present study was aimed at the development of a somoclonal variant and isozyme marker for phyllanthus amarus schum & thonn using inter-nodal segments and the enzyme peroxidase. maximum callus proliferation was obtained on murashige and skoog’s medium supplemented (ms) with 1.0 mg/l of 2,4-dichlorophenoxyacetic acid. three weeks-old pale yellowish white semi-friable callus was used for o...

background: withania somnifera l. dunal, (ashwagandha) belongs to the family solanaceae and is known as ‘indian ginseng’ in the traditional system of medicine. objectives: in the present investigation, comparative analysis was carried out between two different ecotypes of w. somnifera related to total soluble protein and antioxidant enzyme activity [catalase (cat), superoxide dismutase (sod) an...

Three isozymes of catechol 1,2-dioxygenase (pyrocatechase) from Pseudomonas at-villa C-l were separated using DEAE-Toyopearl chromatography. The specific activities of each isozyme were similar to one another. The molecular weights of isozymes 1, 2, and 3 were estimated to be approximately 67,000,64,000, and 59,000, respectively, from gel filtration. On sodium dodecyl sulfate-polyacrylamide gel...

The specific activities and synthesis of the ubiquitous isozyme, PGK-1, and the testis-specific isozyme, PGK-2, have been quantitated and localized in spermatogenic cells of the mouse. There is a fivefold increase in total PGK specific activity between immature and adult testes which begins at approximately 30 days of age, coincident with the appearance of late-middle stage spermatids. The incr...

Two isozymes of horse liver aldehyde dehydrogenase (aldehyde, NAD oxidoreductase (EC 1.2.1.3)), F1 and F2, have been purified to homogeneity using salt fractionation followed by ion exchange and gel filtration chromatography. The specific activities of the two isozymes in a pH 9.0 system with propionaldehyde as substrate were approximately 0.35 and 1.0 mumol of NADH/min/mg of protein for the F1...

In mammalian cells, phosphatidylinositol 4-phosphate (PI4P) 5-kinase (PIP5K) generates phosphatidylinositol 4,5bisphosphate (PI4,5P2) by catalyzing the phosphorylation of PI4P at the D5 position of the inositol ring (Fig. 1) and plays important roles in a wide variety of signal transduction systems through its product PI4,5P2. To date, three mammalian PIP5K isozymes, a , b and g , and three spl...

Using yeast strains with null mutations in structural genes which encode delta-aminolevulinic acid synthetase (HEM1), isozymes of 3-hydroxy-3-methylglutaryl coenzyme A (HMG1 and HMG2), squalene epoxidase (ERG1), and fatty acid delta 9-desaturase (OLE1), we were able to determine the effect of hemes, sterols, and unsaturated fatty acids on both sterol production and the specific activity of 3-hy...

chamomile serves as one of the most important medicinal plants. the most known secondary metabolites in chamomile species are volatile oils of triponoides, poly stilens, flavonoids and phenolic cafeic acid (krori). these metabolites have wide variety of applications thanks to having medicinal properties of anti-inflammation, antispasmodic and bactericide activity. also it is used to cure liver ...

Creatine kinase activity is associated with different isozyme species. We have examined two of these: the cytoplasmic brain (B) isozyme that is expressed in many tissues and is reported to be induced by estrogen and the developmentally regulated cytoplasmic muscle (M) isozyme that is found predominantly in differentiated muscle tissue. Recently, we cloned and sequenced the cDNA for the M isoenz...