Three antioxidant isoflavonoids characterized as 4',7,8-trihydroxyisoflavone (1), 3',4',7-trihydroxyisoflavone (2) and 8-chloro-3',4',5,7-tetrahydroxyisoflavone (3) were isolated from the cultured broth of Streptomyces sp. OH-1049. Among them, 3 is a novel isoflavonoid possessing a chlorine atom in the molecule. In in vitro studies, these antibiotics were found to possess antioxidant activity w...

Centrosema, Phytoalexins, Isoflavones, Tectorigenin, Aesculetin, Chemotaxonomy A phytoalexin isolated from the fungus-inoculated leaflets of Centrosema haitiense, C. pubescens (3 accessions) and C. virginianum has been identified as 5,7,4'-trihydroxy-6-methoxyisoflavone (tectorigenin). This compound is variously accompanied by other isoflavones includ­ ing the previously described phytoalexin, ...

OBJECTIVE In this study, our working hypothesis was that continuous light and fungal elicitation treatment of legume seedlings would lead to enhanced levels of isoflavonoids and soluble proteins. RESULTS Based on short-term light and dark treatments, isoflavonoid (genistein, genistin, daidzein, and daidzin) and soluble protein concentrations were significantly upregulated in the "light" envir...

Novel bioactive components have greatly attracted attention as they demonstrate health benefits. Reversed-phase high performance liquid chromatography (RP-HPLC) showed that isoflavonoid compounds of kudzu root (Pueraria lobata) fermented by Eurtotium cristatum and extracted using de-ionized water were higher active compared with non-fermented. A model of H₂O₂-inducd cell damage was built using ...

Suspension cultures of Pueraria tuberosa, a woody legume, have been established and using different concentrations of growth regulators, sucrose, ammonium and nitrate nitrogen, attempts have been made to improve their isoflavonoid content. The cell cultures grew well on all the treatments. Up to approximately 8 folds increased isoflavonoids content was recorded in the cultures grown in MS mediu...