Isoelectric points of Kunitz' crystalline soybean trypsin inhibitor (STl), Bowman-Birk STI, and STI prepared by chromatography on diethylaminoethyI (DEAE)-cellulose were investigated by isoelectric focusing, gel filtration, ion-exchange resin treatment, solubility test, and titration. Results suggest that the STI of Kunitz may have an isoelectric point below pH 4.5. The isoelectric focusing pat...

The state in which a protein substance exists depends upon the nature of its combination with acids or bases and is changed by change in the protein compound. The nature of the compound of a protein that exists at any hydrogen ion concentration can be ascertained if the isoelectric point of the protein is known. Accordingly information regarding the isoelectric points of vegetable proteins is o...

Despite the significant advantages of capillary isoelectric focusing over gel isoelectric focusing, widespread acceptance of the technique as a replacement for gel isoelectric focusing has not occurred. Several factors contribute to capillary isoelectric focusing’s slower-than-expected adoption; perhaps the most important are the performance and procedural difficulties introduced when capillary...