The synthesis of fluorescent unnatural amino-acid Anap was optimized and the Anap was incorporated into four sites in an acid-pocket or a transmembrane region of human acid-sensing ion channel-1a (hASIC1a). Combinational Anap fluorescence spectra and patch-clamp electrophysiology data illustrated site-specific conformational responses upon toxin mambalgin-1 binding. This combinational approach ...

A new approach to potentiometric sensing utilizing paper-based microfluidic sampling is studied in this work. A solid-contact ion-selective electrode and a solid-contact reference electrode are pressed against a filter paper into which the sample solution is absorbed. The filter paper acts simultaneously as a sampling unit and as a sample container during potentiometric sensing. The paper subst...

Accurate measurements of soil macronutrients (i.e., nitrogen, phosphorus, and potassium) are needed for efficient agricultural production, including site-specific crop management (SSCM), where fertilizer nutrient application rates are adjusted spatially based on local requirements. Rapid, non-destructive quantification of soil properties, including nutrient levels, has been possible with optica...

Abstract Prior studies show that coordinating supply chain inventories with common replenishment epochs (CRE) provides benefit for vendor and buyers. Coordination using selective discount (SD) strategy allows some buyers to participate in the coordination scheme while other buyers continue to order as earlier. This can reduce the vendor’s total cost more than that of all buyers participating an...

Sol-gel and polymeric membrane electrodes, based on thiacalix[4]arene as a neutral carrier, weresuccessfully developed for the detection of VO2+ in aqueous solutions. The sol-gel and PVCmembrane electrodes exhibited linear response with Nernstian slopes of 29.3 and 28.4 mV perdecade, respectively, within the vanadyl ion concentration ranges from 1.0 × 10-6 to 1.0 × 10-1mol dm-3 and from 1.0 × 1...

Enzyme sensor for organophosphorus insecticides organophosphorus hydrolase in a polymer membrane on a pH sensor is used to catalyse the hydrolysis of compounds with phoshate ester functionality. The detected [H+] change is proportional to the concentration of the phosphate ester insecticide. This is a good example of the use of an enzyme to give selectivity to a specific class of compounds. Unf...

AIM The aim of the present study was to evaluate the relationship of drinking water fluoride levels with children's intelligence quotient (IQ). MATERIALS AND METHODS Water was collected from initially identified endemic fluoride regions according to the geological research of Government of India. Fluoride concentration of the water was assessed by utilizing fluoride ion selective electrode, O...

OBJECTIVE To test the hypothesis that glucose ingestion leads to a decrease in plasma ionized calcium (iCa) and ionized magnesium (iMg) during the third trimester of pregnancy. METHODS We studied 54 women who underwent a 50 g glucose challenge test (GCT) and 27 women who underwent a 3-hour 100 g glucose tolerance test (GTT) because of an abnormal GCT. Plasma glucose was measured with an autom...

We studied the stability and linearity of the AVL and Nova Mg and Ca ion-selective electrodes and the relation between the ionized Ca and ionized Mg results reported by each analyzer. The response of the electrodes to different concentrations of Mg and Ca was determined for saline solutions, aqueous solutions, and serum samples. The electrodes from both manufacturers demonstrated acceptable sta...