Radiolabeled annexin V (ANV) has been widely used for imaging cell apoptosis. Recently, a novel ANV-Kunitz-type protease inhibitor fusion protein, ANV-6L15, was found to be a promising probe for improved apoptosis detection based on its higher affinity to phosphatidylserine (PS) compared to native ANV. The present paper investigates the feasibility of apoptosis detection using radioiodinated AN...

Despite the use of multimodal treatments incorporating surgery, chemotherapy and radiotherapy, local control of gliomas remains a major challenge. The potential of a new treatment approach called indirect radio-chemo-beta therapy using the synergy created by combining methotrexate (MTX) with bromodeoxyuridine (BrUdR) under optimum energy x-ray irradiation is assessed. 9L rat gliosarcoma cells p...

BACKGROUND A case of congenital hypothyroidism with goiter (CHG) in a juvenile French bulldog was identified and hypothesized to be caused by dyshormonogenesis of genetic etiology. OBJECTIVES To describe case management, unusual phenotypic aspects, and a CHG-causing mutation in a French bulldog. ANIMALS Thyroid tissue and blood from a CHG-affected French bulldog and 4 normal control dogs an...

Although there is abundant published evidence that radioiodinated antigens interfere in digoxin radioimmunoassays, other radioimmunoassays are similarly affected. We investigated the relationship of radioiodinated antigen structure to its function in the immunoassay. Carrier-free 125I-labeled iodotyrosine and iodohistamine derivatives were incubated with human serum, and the bound and free frac...

Design and development of new approaches for targeted radiotherapy of cancer and improvement of therapeutic index by more local radiation therapy are very important issues. Adenovirus-mediated delivery of the sodium iodide symporter (NIS) gene to cancer cells is a powerful technique to concentrate lethal radiation in tumor cells and eradicate tumors with increased therapeutic index. A replicati...

introduction: effective debridement of the root canal system with chemical irrigants prior to obturation is the key to long-term success of endodontic therapy. the purpose of this study is to compare the antibacterial activity of 2.5% sodium hypochlorite (naocl) and 2% iodine potassium iodide (iki) solutions as intracanal disinfectant in infected root canals during one-visit endodontic treatmen...

an experiment was conducted to investigate the effect of thyroid gland activity modulation (hyper or hypothyroidism) on energy utilization in broiler chickens. two hundred and forty one-day-old cobb broiler chicks were distributed into three dietary metabolizable energy (me) treatment groups (80 chicks each). the control group (e0) was fed basal diet (3150 kcal/kg diet), the second group (e1) w...

A new method is described for the detection of variations in the release rate of all iodinated products by the thyroid gland in man. (125)I was employed to endogenously label the thyroid gland, and a parenterally administered (131)I thyroxine dose was used to generate a (131)I reference source. Thyroidal iodine release activity was quantitatively assessed by the measurement of variations in (12...

Thyroid hormones are essential for normal development and metabolism. Thyroid hormone biosynthesis requires iodide uptake into the thyrocytes and efflux into the follicular lumen, where it is organified on selected tyrosyls of thyroglobulin. Uptake of iodide into the thyrocytes is mediated by an intrinsic membrane glycoprotein, the sodium-iodide symporter (NIS), which actively cotransports two ...

The highly toxic curraremimetic and cholinergic neuron marker (-)-5-iodobenzovesamicol (IBVM) has been labeled with iodine-125 and iodine-123. [125I]IBVM, suitable for animal distribution and ex vivo autoradiographic studies, was synthesized by solid-state exchange; isolated yields were 65-89% with specific activities in the range of 130-200 Ci/mmol. The synthesis of no-carrier-added (-)-5-[125...