An organ-specific invertase deficiency affecting only the primary root system is described in the Oh 43 inbred maize (Zea mays). Invertases (acid and neutral/soluble and insoluble) were assayed in various tissues of hybrid (NK 508) and inbred (Oh 43, W 22) maize lines to determine the basis for an early report that Oh 43 root tips were unable to grow on sucrose agar (27). Substantial acid inver...

To distinguish their roles in early kernel development and stress, expression of soluble (Ivr2) and insoluble (Incw2) acid invertases was analyzed in young ovaries of maize (Zea mays) from 6 d before (-6 d) to 7 d after pollination (+7 d) and in response to perturbation by drought stress treatments. The Ivr2 soluble invertase mRNA was more abundant than the Incw2 mRNA throughout pre- and early ...

Phytohemagglutinin (PHA), the seed lectin of the common bean, accumulates in protein storage vacuoles of storage parenchyma cells in cotyledons. When expressed in yeast, PHA is efficiently targeted to the yeast vacuole [Tague and Chrispeels (1987). J. Cell Biol. 105, 1971-1979]. To identify vacuolar sorting information in PHA, a series of 3' deletions of the PHA gene were fused in-frame to a tr...

Aim of present study was production, purification and characterization of invertase by Aspergillus niger (isolated from soil) using fruit peel waste as a substrate. Screening of various fungi grown on potato dextrose-agar (PDA) was done using liquid medium of 50% sucrose-Cazpek. After incubation at 28 ̊C for 10 days, the invertase activity was determined in the culture filtrate using Fehling’s s...

A wheat stay-green mutant, tasg1, was observed to exhibit significantly delayed senescence in the late developmental stage. The photosynthetic capacity of the flag leaf was greater in tasg1 than in wild type (WT) plants. In addition, the grain volume of tasg1 was significantly higher than that of WT at the early filling stage. The content of various cytokinins (CKs) in the grain was significant...

An in vitro translocation system has been reconstituted with subcellular fractions from the cell wall-less mutant of Neurospora crassa (fz;sg;os-1). Prepro alpha factor and invertase, secretory proteins from yeast, were faithfully translocated and glycosylated by Neurospora microsomes when presence cotranslationally in the Neurospora translation system. When presence cotranslationally in the Ne...

We have studied the molecular physiology of photosynthate unloading and partitioning during seed development of fava bean (Vicia faba). During the prestorage phase, high levels of hexoses in the cotyledons and the apoplastic endospermal space are correlated with activity of cell wall-bound invertase in the seed coat. Three cDNAs were cloned. Sequence comparison revealed genes putatively encodin...

background: in algeria the date wastes production is estimated at least 85.000 tones. date wastes is an economic source of carbohydrates for conversion to industrial enzymes because it is readily available and relatively low priced.objective: the aim of the present study was to investigate the potential of using date wastes as a substrate for the production of a-amylase and invertase. materials...

Streptomyces sp. ALKC8 produced high levels of extra-cellular invertase (350 Ul) after incubation for 24 h at pH 5.0, temperature 37°C in the presence of sucrose (3%). The optimum temperature and pH for enzyme activity were 60°C and 5.0 respectively. All metal ions except Na, Hg and Ca were well tolerated and did not adversely affect invertase activity. However, SDS inhibited the enzyme activit...