Infertility is one of the most common problems worldwide. Half causes infertility are due to women and half men. For this reason, studies overcome problem concentrated on way ensuring continuation generation by using high technology. purpose, applications such as (in vitro fertilization) IVF (intra cytoplasmic sperm injection) ICSI widely applied. This process done experts produces non-objectiv...

background: irradiation is one of the major causes of induced sperm dna damage. various studies suggested a relation between sperm dna damage and fertilization rate after intra-cytoplasmic sperm injection (icsi). objective: in this study, fertilization rate and premature chromosome condensation (pcc) formation after icsi of hamster oocytes with irradiated sperms from normal and oligosperm indiv...

Single-cell pulsed-field gel electrophoresis (SCPFGE) with dual electrode pairs was developed to detect the early stage of DNA fragmentation in human sperm. The motile sperm were purified by the commonly used density-gradient centrifugation technique and subsequent swim-up. The sperm were embedded in a thin film of agarose containing bovine trypsin (20 µg/mL) and were then lysed. Prior to SCPFG...

There is an increased risk of thromboembolism, anticoagulant-related hemorrhage, fetal-wastage and congestive cardiac failure in pregnant women with mechanical heart valves. In order to have a good outcome, the care of such patients must necessarily be multidisciplinary and in a well- equipped centre with adequate support services .One such patient who had mechanical mitral and aortic valves re...

objective: selection of sperm for intra-cytoplasmic sperm injection (icsi) is usually considered as the ultimate technique to alleviate male-factor infertility. in routine icsi, selection is based on morphology and viability which does not necessarily preclude the chance injection of dna-damaged or apoptotic sperm into the oocyte. sperm with high negative surface electrical charge, named “zeta ...

Assisted reproduction with testicular sperm extraction (TESE) and intra-cytoplasmic sperm injection (ICSI) are fertility treatment options for couples with severe oligospermia or azoospermia. A retrospective review was performed of 146 TESE procedures in a specialist fertility centre in Ireland. The indication for TESE was obstructive azoospermia (OA) in 59% (n = 80) and non-obstructive azoospe...

Objective Selection of sperm for intra-cytoplasmic sperm injection (ICSI) is usually considered as the ultimate technique to alleviate male-factor infertility. In routine ICSI, selection is based on morphology and viability which does not necessarily preclude the chance injection of DNA-damaged or apoptotic sperm into the oocyte. Sperm with high negative surface electrical charge, named “Zeta p...

Obesity in men of reproductive age is globally on the increase. There is clear evidence from epidemiological studies that obesity impacts negatively on male fertility; it is associated with hypogonadism, although it is less consistently linked to impaired spermatogenesis and tests of sperm function, including DNA fragmentation. Sperm from obese men used for in vitro fertilisation/intra cytoplas...