The objective of this research was to investigate the influence of the over-expression of recombinant interferon-g during high cell density cultivation on cellular characteristics of recombinant E. coli. Batch and fed-batch culture techniques were employed to grow Escherichia coli BL21 for production of human gamma-interferon in pET expression system. Final cell densities in batch and fed-batch...

Background and Objective: Due to the severe inflammatory conditions and cytokine storm in COVID-19 disease, corticosteroids are used worldwide as adjunctive therapy for these patients due to their anti-inflammatory effects. However, due to limited and inconsistent information about the effectiveness of this drug, this study aimed to investigate the benefit of combined use of β-interferon and me...

interferons (ifns) are a family of small regulatory glycoproteins that play a central role in the defense against viral infections. although ifns have been initially discovered as antiviral factors, today they are known as an integral part of the cytokine network that affect a wide range of biological processes. ifns exert their pleiotropic effects through their multisubunit cell surface recept...

bronchiolitis has been known as one of the pathological features of lung injuries in mustard gas exposed patients the purpose of this research was to evaluate the efficacy of interferon gamma-1b on the lung function in mustard gas exposed patients with bronchiolitis. in multicenter research interferon gamma-1b was effective in pulmonary fibrosis with unknown reason, but assessment of effect of ...

results overall, 3 (15%) subjects achieved svr, 10 cases (50%) relapsed after treatment cessation and 7 (35%) patients did not clear hdv during the treatment. conclusions hdv coinfection with hbv had very low response rate to high doses and long durations of ifn α-2b therapy. objectives we aimed to determine the efficacy of ifn α-2b therapy in patients with hbv/hdv coinfection. patients and met...

introduction: the aim of this study is to assess the therapeutic efficacy and safety of a particular brand of ifn alpha (pdferon b®) in combination with ribavirin (rlba) on iranian patients with chronic hepatitis c (chc). the addition of riba to the standard treatment with interferon (ifn) alpha led to an improvement in sustained virologic response (svr) from less than 20% with ifn monotherapy ...

background: the refolding of proteins from inclusion bodies is affected by several factors, including solubilization of inclusion bodies by denaturants, removal of the denaturant, and assistance of refolding by small molecule additives. objectives: the purpose of this study was optimization of recombinant human interferon-b purification in order to achieve higher efficiency, yield, and a produc...

none of the approved immunomodulatory drugs in adults multiple sclerosis (ms) patients have been officially approved for the pediatric patients and are currently used off-label in this population. in this study, we evaluated the effectiveness and tolerability of intramuscular interferon beta1-a (avonex®) and subcutaneously injected interferon beta1-b (betaferon®) in children with definite relap...

background: it has been reported that liver function alterations occur in 8–38% of patients with relapsing-remitting multiple sclerosis (rrms) treated with interferon-beta in controlled clinical trials or in prospective but non-controlled studies. we report herein a case of symptomatic liver dysfunction associated with two-year-period of regular interferon-beta-1a (avonex®) intramuscular inject...