A set of 16 expressed sequence tag (EST)-derived simple sequence repeat (SSR) and 15 EST-derived single nucleotide polymorphism (SNP) markers together with 4 amplified fragment length polymorphism (AFLP) primer combinations were analyzed on 43 wild (Hordeum vulgare ssp. spontaneum – HS), 35 cultivated (H. vulgare ssp. vulgare – HV) and 12 elite (H. vulgare ssp. vulgare – from EU) barley lines. ...

In this study, we evaluated three PCR-based methods for the molecular typing of nonpathogenic Fusarium oxysporum isolates: random amplified polymorphic DNA (RAPD), polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and amplified fragment length polymorphism (AFLP). The analyses were performed using 64 isolates of F. oxysporum collected from cotton-producing areas in E...

Malassezia yeasts are associated with several dermatological disorders. The conventional identification of Malassezia species by phenotypic methods is complicated and time-consuming, and the results based on culture methods are difficult to interpret. A comparative molecular approach based on the use of three molecular techniques, namely, amplified fragment length polymorphism (AFLP) analysis, ...

MMPs have significant role in pathological processes and are associated with cancer invasion, and metastasis, cartilage destruction in arthritis. Non-synonymous single nucleotide polymorphisms (nsSNPs) occurring in protein coding regions of MMP potentially affect their function, and results in diseases. In this study we performed an insilco analysis of SNPs associated with MMP2 genes. Total of ...

BACKGROUND Neonectria ditissima is one of the most important fungal pathogens of apple trees, where it causes fruit tree canker. Information about the amount and partitioning of genetic variation of this fungus could be helpful for improving orchard management strategies and for breeding apple cultivars with high levels of genetically determined resistance. In this study single-spore Neonectria...

Abstract In recent years, tan spot of wheat caused by the fungus Pyrenophora tritici-repentis has become more prevalent in Oklahoma. Experiments were conducted to investigate race structure, disease symptoms and genetic variability P. isolates collected from winter over three decades. Race determination was for 16 based on expression necrosis and/or chlorosis produced differentials. Variability...

The development of molecular techniques, in particular the use of molecular markers have been used to identify markers linked to useful traits and made great advances in recent years. Amplified fragment length polymorphism (AFLP) analysis was used to analyze the geographical differentiation, phylogenetic relationships and to identify molecular markers linked to taro leaf blight resistance genes...

Candida parapsilosis former groups II and III have recently been established as independent species named C. orthopsilosis and C. metapsilosis, respectively. In this report, 400 isolates (290 patients) previously classified as C. parapsilosis by conventional laboratory tests were screened by BanI digestion profile analysis of the secondary alcohol dehydrogenase gene fragment and by amplificatio...

Forty-four soybean genotypes with different photoperiod response were selected after screening of 1000 soybean accessions under artificial condition and were profiled using 40 SSR and 5 AFLP primer pairs. The average polymorphism information content (PIC) for SSR and AFLP marker systems was 0.507 and 0.120, respectively. Clustering of genotypes was done using UPGMA method for SSR and AFLP and c...

little information is available regarding genetic variation in wild wheat relatives from iran. in this study, genetic diversity of 36 populations of wild einkorn wheat, triticum boeoticum, was studied using amplified fragment length polymorphism (aflp) primer combinations. seventeen aflp primer combinations led to amplify 979 scorable fragments ranging from 50 to 500 bp and of these, 429 (44%) ...