Diisopropylphosphorofluoridate reacts with trypsinogen and chymotrypsinogen and inhibits the potential activity of both zymogens. The reactions follow pseudo first-order kinetics and proceed approximately four orders of magnitude slower than diisopropylphosphorylation of the corresponding enzymes. Correlation of initial rates of inactivation with incorporation of the reagent indicates that zymo...

Bacterial α-amylase was immobilized in sol-gel matrices using different techniques and precursors (i.e. tetraethoxysilane and methyltriethoxysilane). Sol-gel entrapment procedures were adjusted to enhance the immobilized enzyme activity and the textural properties of the silica matrices. The addition of a template agent, the variation of aging and drying conditions or the gelation performed in ...

Macromolecular networks consisting of homogeneously distributed covalently bonded inorganic and organic precursors are anticipated to show remarkable characteristics, distinct from those of the individual constituents. A novel hyper-cross-linked ultrathin membrane is presented, consisting of a giant molecular network of alternating polyhedral oligomeric silsesquioxanes and aromatic imide bridge...

The splicing of messenger RNA precursors in vitro proceeds through an intermediate that has the 5' end of the intervening sequence joined to a site near the 3' splice site. This lariat structure, which has been characterized for an adenovirus 2 major late transcript, has a branch point, with 2'-5' and 3'-5' phosphodiester bonds emanating from a single adenosine residue. The excised intervening ...

Protein turnover occurs during differentiation of Azotobacter vinelandii 12837 to the extent of 50% during encystment and 7% during germination. The addition of rifampin at the initiation of encystment prevents encystment and inhibits turnover. In germinating cysts, protein turnover is essential owing to an apparent lack of certain amino acid biosynthetic enzymes. The capacity to synthesize sul...

A key bottleneck for material discovery is synthesis. While significant advances have been made in computational design, synthesis pathways are still often determined through trial and error. In this work, we develop a method that predicts the major product of solid-state reactions. The main advance presented here construction fixed-length, learned representations Precursors represented as node...

Among the metabolic events which have been found to accompany the ingestion of particles by polymorphonuclear leukocytes (1) is an enhanced incorporation of inorganic phosphate-P32 into the total phosphatide fraction (2). Under suitable conditions, there was found to be a doubling of the specific activity of this fraction in leukocytes which were engulfing starch granules, compared with leukocy...

We report the formation mechanism of FePt nanoparticles (NPs) by a high temperature polyol method using an equimolar ratio of Fe and Pt-precursor with different Pt-precursors. Pt(acac)2, PtCl2, PtCl4 and H2PtCl6·H2O were used as Pt-precursors and Fe(acac)3 as the only Fe-precursor. Different stoichiometric compositions along with variation in size were obtained by using different precursors of ...