Many viruses, including human influenza A virus, have developed strategies for counteracting the host type I interferon (IFN) response. We have explored whether avian influenza viruses were less capable of combating the type I IFN response in mammalian cells, as this might be a determinant of host range restriction. A panel of avian influenza viruses isolated between 1927 and 1997 was assembled...

Heterotypic immunity to influenza virus in ferrets operated against heterotypic influenza viruses but not heterologous viruses. Contrary to prior reports, the protection conferred lasted for at least 18 months. This type of immunity limited virus shedding but did not prevent infection. These results suggest that this phenomenon could play a role in determining the severity of infections caused ...

Annual influenza epidemics are caused not only by influenza A viruses but also by influenza B viruses. Initially established for the generation of recombinant influenza A viruses, plasmid-based reverse genetics techniques have allowed researchers the generation of wild type and mutant viruses from full-length cDNA copies of the influenza viral genome. These reverse genetics approaches have allo...

Immunochromatography (IC) is an antigen-detection assay that plays an important role in the rapid diagnosis of influenza virus because the protocol is short time and easy to use. Despite the usability of IC, the sensitivity is approximately 10(3) pfu per reaction. In addition, antigen-antibody interaction-based method cannot be used for the detection of influenza viruses with major antigenic ch...

influenza a viruses possesses two virion surface glycoproteins including haemagglutinin (ha) and neuraminidase (na). the na plays an important role in viral replication and promotes virus release from infected cells and facilitates virus spread throughout the body. to find out any genomic changes that might be occurred on na gene of avian influenza circulating viruses, we have genetically analy...

Influenza viruses cause seasonal epidemics whose intensity varies according to the circulating virus type and subtype. We aim estimate influenza-like illness (ILI) incidence attributable influenza in France from October 2014 May 2019. Physicians participating French Sentinelles network reported number of patients with ILI seen consultation performed nasopharyngeal swabs a sample these patients....

Antisera to the type-specific internal influenza virus matrix (M) protein of a type A influenza virus were produced in goats. In the presence of complement, anti-M serum was cytotoxic for target cells which were infected with a variety of serologically distinct type A influenza viruses, but did not react with type B influenza virus-infected cells. Absorption experiments indicated that anti-M se...

Lateral flow tests also known as Immunochromatography (IC) is an antigen-detection method conducted on a nitrocellulose membrane that can be completed in less than 20 min. IC has been used as an important rapid test for clinical diagnosis and surveillance of influenza viruses, but the IC sensitivity is relatively low (approximately 60%) and the limit of detection (LOD) is as low as 10³ pfu per ...

BACKGROUND Highly pathogenic avian H5N1 influenza viruses preferentially infect alveolar type II pneumocytes in human lung. However, it is unknown whether this cellular tropism contributes to high viral virulence because the primary target cells of other influenza viruses have not been systematically studied. METHODS We provide the first comparison of the replication, tropism, and cytokine in...

Background & Aims: Influenza viruses are important causes of mortality and morbidity in children. The aim of this study was to assess the presence of antibodies (IgG) against Influenza A in children in Kerman, Iran. Methods: In a cross-sectional study, 200 children aged 4-14 years referred to Besaat Clinic and Afzalipour hospital for diseases other than influenza were enrolled. Sera were tested...