Background Cryopreservation of ovarian cortex is increasingly used to preserve fertility before cancer therapy. Recently, studies show that Brain-derived neurotrophic factor (BDNF) may be involved in oocyte maturation. Brain-derived neurotrophic factor (BDNF) is member of neurotrophin family that has anti-apoptotic effects on nervous system. Recent researches show that it also plays key role in...

Melatonin has been used as a supplement in culture medium to improve the efficiency of in vitro produced mammalian embryos. Through its ability to scavenge toxic oxygen derivatives and regulate cellular mRNA levels for antioxidant enzymes, this molecule has been shown to play a protective role against damage by free radicals, to which in vitro cultured embryos are exposed during early developme...

Background This study was undertaken to investigate the efficiency of two different embryo somatic cell co-culture conditions tissue culture medium 199 (TCM199)–vero cells and Menezo B2 (B2)-vero cells for the in vitro developmental quantity and quality of bovine embryos. MaterialsAndMethods Bovine oocytes were allowed to mature and subsequently undergo fertilization in vitro. Their presumptive...

The aim of this investigation was to design and develop nanoemulsions (NEs) as novel ophthalmic delivery systems for brinzolamide (BZD). Phase behavior of quaternary systems composed of triacetin and CapryolTM 90 (selected oils, screened through the solubility studies), various surfactants (namely, Cremophor RH 40, Brij 35, Labrasol and tyloxapol), Transcutol P (as co-surfactant) an...

Cell adhesion molecules have been shown to be important mediators of morphogenesis and pattern formation. In this study, we have shown that N-cadherin is expressed in a specific spatiotemporal manner in the developing limb bud during chondrogenesis in vivo and in cultured limb mesenchyme in vitro. The time period of maximal expression of N-cadherin corresponds to the period of active cellular c...

In the developing chick leg bud, massive programmed cell death occurs in the interdigital region. Previously, we reported the inhibition of cell death by separation of the interdigital region from neighboring digit cartilage. In this study, we examined the relationship between cell death and cartilaginous tissue in vitro. First, cell fate was observed with DiI that was used to examine cell move...

the aim of this investigation was to design and develop nanoemulsions (nes) as novel ophthalmic delivery systems for brinzolamide (bzd). phase behavior of quaternary systems composed of triacetin and capryoltm 90 (selected oils, screened through the solubility studies), various surfactants (namely, cremophor rh 40, brij 35, labrasol and tyloxapol), transcutol p (as co-surfactant) and water a...

the goal of this research is to determine the feasibility of loading rifampin into mesoporous silica nanoparticles. rifampin was selected as a model lipophilic molecule since it is a well-documented and much used anti tuberculosis drug. the mesoporous silica nanoparticles were prepared by using tetraethyl ortho silicate and cetyltrimethyl ammonium bromide (as surfactant); the prepared nanoparti...

Background: Curcumin as a yellow natural compound extracted from turmeric root is known it as an antibacterial agent. One of the nanoparticles ability is to decrease the defects of usual drug delivery systems. Chitosan is a low toxic, biodegradable, biocompatible and safe polymer which is used in production of nanoparticles. Nanoparticles like chitosan-tripolyphosphate (TPP) are able to increas...