Abstract Aims High viscosity, low surface tension and hydrophobicity are specific properties of maize root mucilage which contribute to modulate the spatial configuration liquid phase in soil pores. Several processes rhizosphere, particularly nutrient absorption, exudation microbial activity, may cause strong temporal variations chemistry solution rhizosphere. Although physical have been repeat...

Despite trivalent chromium’s manifold functionalities in industrial manufacturing processes, when it is improperly treated and disposed to the environment, due external environmental factors could get converted hexavalent chromium that has toxic effects on humans our environment. Hence, there a stringent need optimize current practices of handling, management, disposal. This paper puts forth in...

A molecular understanding of prion diseases requires an understanding of the mechanism of amyloid fibril formation by the prion protein. In particular, it is necessary to define the sequence of the structural events describing the conformational conversion of monomeric PrP to aggregated PrP. In this study, the sequence of the structural events in the case of amyloid fibril formation by recombin...

The circular dichroism of the dd-carboxypeptidase-transpeptidase from Streptomyces R61 shows in the near u.v. a set of weak extrema at 289nm (positive) and at 282, 275 and 268nm (all negative). In the far u.v. it shows negative extrema at 217-218 and 208nm, crossover at 202nm and a positive maximum at about 194nm. The u.v. absorption of the enzyme shows it to contain tyrosine and tryptophan in ...

Hydroxyapatite (HA) was prepared from catfish bones, identified as catfish HA (CFHA), using mechanical and chemical treatment methods. CFHA was characterized by x-ray diffraction (XRD) and scanning electron microscope (SEM) techniques to confirm the presence of HA. The ability of CFHA to remove uranium (U(VI)) from aqueous phase was investigated using both batch and column experiments. Adsorpti...

The purpose of this study was to produce a novel pH sensitive hydrogel with superior thermal stability, composed of poly(acrylic acid) (PAA) and cellulose nanocrystal (CNC). CNC was extracted from kenaf fiber through a series of alkali and bleaching treatments followed by acid hydrolysis. PAA was then subjected to chemical cross-linking using the crosslinking agent (N,N-methylenebisacrylamide) ...

The requirements for substrate binding in the quinoprotein glucose dehydrogenase (GDH) in the membranes of Escherichia coli are described, together with the changes in activity in a site-directed mutant in which His262 has been altered to a tyrosine residue (H262Y-GDH). The differences in catalytic efficiency between substrates are mainly related to differences in their affinity for the enzyme....

The equilibrium constant of the phosphoglyceromutase reaction was determined over a range of pH (5.4-7.9), in solutions of different ionic strength (0.06-0.3) and in the presence of Mg(2+), at 30 degrees C and at 20 degrees C. The values obtained (8.65-11.65) differ substantially from previously published values. The third acid dissociation constants were redetermined for 2- and 3-phosphoglycer...

Changes in the configuration of proteins were studied by the modifications of the ultraviolet absorption of their alkaline solutions. These were expressed in terms of the ratio O.D.(pH12)/O.D.(pH7), termed side-group ionization ratio (SGIR). This ratio showed two peaks; one at 300 to 305 mmicro is known to correspond to the phenolic hydroxyl of tyrosine and another at 245 mmicro seems to be cau...

Transient kinetic studies of the reversible oxidative phosphorylation of d-glyceraldehyde 3-phosphate catalysed by d-glyceraldehyde 3-phosphate dehydrogenase show that all four sites of the tetrameric lobster enzyme are simultaneously active, apparently with equal reactivity. The rate-determining step of the oxidative phosphorylation is NADH release at high pH and phosphorolysis of the acyl-enz...