نتایج جستجو برای: in his post

تعداد نتایج: 17046829  

Journal: :Biosensors & bioelectronics 2010
Erman Timurdogan Natali Ozber Sezin Nargul Serhat Yavuz M Salih Kilic I Halil Kavakli Hakan Urey B Erdem Alaca

Label-free detection of the interaction between hexahistidine-tagged human κ-opioid receptor membrane protein and anti-His antibody is demonstrated in liquid by an optical microelectromechanical system utilizing electromagnetically actuated microresonators. Shift in resonance frequency due to accretion of mass on the sensitive surface of microresonators is monitored via an integrated optical re...

Journal: :Organic & biomolecular chemistry 2016
Chih-Chi Chu Scott K Silverman

We evaluate the ability of hexahistidine (His6) tags on peptide and protein substrates to recruit deoxyribozymes for modifying those substrates. For two different deoxyribozymes, one that creates tyrosine-RNA nucleopeptides and another that phosphorylates tyrosine side chains, we find substantial improvements in yield, kobs, and Km for peptide substrates due to recruiting by His6/Cu(2+). Howeve...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد گرمسار - دانشکده زبانهای خارجی 1389

abstract this study examines the effect of teaching lexical inferencing strategies on developing reading comprehension skill of iranian advanced efl learners. participants were female students of meraj and shokouh institudes of garmsar a quasi-experimental design using two intact advanced classes of efl students at meraj and shokouh institutes. as the first step, a general toefl proficiency te...

Journal: :Langmuir : the ACS journal of surfaces and colloids 2014
Gabriel S Longo Monica Olvera de la Cruz Igal Szleifer

We present a molecular theory to study the adsorption of different species within pH-sensitive hydrogel nanofilms. The theoretical framework allows for a molecular-level description of all the components of the system, and it explicitly accounts for the acid-base equilibrium. We concentrate on the adsorption of hexahistidine, one of the most widely used tags in bio-related systems, particularly...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد تهران مرکزی - دانشکده ادبیات و زبانهای خارجی 1390

august wilson is undoubtedly one of the rare black playwrights whose works have attracted streams of attention and worldwide audience. the present study aims at analyzing fences and piano lesson which are two of the most successful plays in his pittsburgh cycle from the perspective of michel foucault’s theories and ideas. studying these two plays from foucault’s perspective opens new windows in...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شیراز - دانشکده علوم انسانی 1391

tennessee williams (1911-1983), the modern american dramatist, had his own unique school of dramaturgy. the dramas which he depicted are populated by characters who are lonely, desperate, in anxiety, alienated and in one word lost. they face challenges which they may overcome or not, through the choices they make. all these moods and conditions are clearly seen and explained in the theory of ex...

Journal: :Acta crystallographica. Section F, Structural biology and crystallization communications 2007
Akari Shinohara Mayuko Sakuma Toshiharu Yakushi Seiji Kojima Keiichi Namba Michio Homma Katsumi Imada

The polar flagellum of Vibrio alginolyticus is rotated by the sodium motor. The stator unit of the sodium motor consists of four different proteins: PomA, PomB, MotX and MotY. MotX and MotY, which are unique components of the sodium motor, form the T-ring structure attached to the LP ring in the periplasmic space. MotY has a putative peptidoglycan-binding motif in its C-terminal region and MotX...

2011
Ryan Choi Angela Kelley David Leibly Stephen Nakazawa Hewitt Alberto Napuli Wesley Van Voorhis

The recombinant expression of soluble proteins in Escherichia coli continues to be a major bottleneck in structural genomics. The establishment of reliable protocols for the performance of small-scale expression and solubility testing is an essential component of structural genomic pipelines. The SSGCID Protein Production Group at the University of Washington (UW-PPG) has developed a high-throu...

Journal: :BMC Biotechnology 2003
Christoph Scheich Volker Sievert Konrad Büssow

BACKGROUND Functional Genomics, the systematic characterisation of the functions of an organism's genes, includes the study of the gene products, the proteins. Such studies require methods to express and purify these proteins in a parallel, time and cost effective manner. RESULTS We developed a method for parallel expression and purification of recombinant proteins with a hexahistidine tag (H...

Journal: :Nanoscale 2013
Dris Ihiawakrim Ovidiu Ersen Frédéric Melin Petra Hellwig Izabela Janowska Dominique Begin Walid Baaziz Sylvie Begin-Colin Cuong Pham-Huu Rachid Baati

A practically simple top-down process for the exfoliation of graphene (GN) and few-layer graphene (FLG) from graphite is described. We have discovered that a biocompatible amphiphilic pyrene-based hexahistidine peptide is able to exfoliate, functionalize, and dissolve few layer graphene flakes in pure water under exceptionally mild, sustainable and virtually innocuous low intensity cavitation c...

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