Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...

BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

The function of pslD, which is part of the psl operon from Pseudomonas aeruginosa, was investigated in this study. The psl operon is involved in exopolysaccharide biosynthesis and biofilm formation. An isogenic marker-free pslD deletion mutant of P. aeruginosa PAO1 which was deficient in the formation of differentiated biofilms was generated. Expression of only the pslD gene coding region resto...

This is a review of the book Rabindranath Tagore’s Journey as an Educator: Critical Perspectives on His Poetics and Praxis, edited by Mohammad A. Quayum, published Routledge, UK

Label-free detection of the interaction between hexahistidine-tagged human κ-opioid receptor membrane protein and anti-His antibody is demonstrated in liquid by an optical microelectromechanical system utilizing electromagnetically actuated microresonators. Shift in resonance frequency due to accretion of mass on the sensitive surface of microresonators is monitored via an integrated optical re...

We evaluate the ability of hexahistidine (His6) tags on peptide and protein substrates to recruit deoxyribozymes for modifying those substrates. For two different deoxyribozymes, one that creates tyrosine-RNA nucleopeptides and another that phosphorylates tyrosine side chains, we find substantial improvements in yield, kobs, and Km for peptide substrates due to recruiting by His6/Cu(2+). Howeve...

BĂĄsnĂ­k a spisovatel VĂ­tězslav Nezval (1900–1958), vĹŻdčí osobnost československĂŠ mezivĂĄlečnĂŠ uměleckĂŠ avantgardy zĂĄroveň přísluĹĄnĂ­k komunistickĂŠho hnutĂ­, v srpnu 1934 navĹĄtĂ­vil Moskvu jako jeden ze zĂĄpadnĂ­ch hostĹŻ ustavujĂ­cĂ­ho sjezdu Svazu sovětskĂ˝ch spisovatelĹŻ nĂĄsledujĂ­cĂ­m roce vydal prozaicko-esejistickou reflexi tohoto pobytu s nĂĄzvem NeviditelnĂĄ ...

Abstract This article examines the contributions of Ghulām ʿAlī “Āzād” Bilgrāmī (1704–1786) to our understanding comparative poetics and Arabic in eighteenth-century Hindustan. It attends Azad’s oeuvre through lenses translation, multilingualism, literary science. Philological analysis reveals how Azad establishes analogues across these three languages that attest adaptive capacity poetics. His...

We present a molecular theory to study the adsorption of different species within pH-sensitive hydrogel nanofilms. The theoretical framework allows for a molecular-level description of all the components of the system, and it explicitly accounts for the acid-base equilibrium. We concentrate on the adsorption of hexahistidine, one of the most widely used tags in bio-related systems, particularly...