pr81 is a monoclonal antibody that binds with high affinity to muc1 that over expressed on breast tumors. pr81 is considered a suitable targeting molecule that was radiolabeled using cu-64 for positron imaging studies. the monoclonal antibody was conjugated with dota moiety and after purification was evaluated for radiochemical purity, immunoreactivity, cell toxicity and structure integrity as ...

The identification of human tumor antigens has potential utility in the diagnosis and treatment of cancers. We demonstrate here a complete strategy to profile immunoreactivity and identify tumor antigens from proteins derived from tumor cell lines. Microarrays of proteins produced from 2-D LC fractionation of prostate tumor cell-line lysates were used to profile immunoreactivity in the sera of ...

The present paper shows by means of confocal laser scanning microscopy the immunoreactivity of rat cerebellar Lugaro cells for calbindin, synapsin-I, PSD-95, GluR1, CaMKII alpha, and N-cadherin. Lugaro cells were easily characterized by their location beneath Purkinje cells. Calbindin revealed immunoreactivity in the cell body, and the axonal and dendritic processes. Synapsin-I labelled the pre...

Inhibitors of DNA-binding/differentiation (ID) proteins bind to basic helix‑loop‑helix (bHLH) transcription factors, including those that regulate differentiation and cell‑cycle progression during development, and regulate gene transcription. However, little is known about the role of ID proteins in the brain under transient cerebral ischemic conditions. In the present study, we examined the ef...

Recent evidence exists that glucose transporter 3 (GLUT3) plays an important role in the energy metabolism in the brain. Most previous studies have been conducted using focal or hypoxic ischemia models and have focused on changes in GLUT3 expression based on protein and mRNA levels rather than tissue levels. In the present study, we observed change in GLUT3 immunoreactivity in the adult gerbil ...

p63, a p53 homologue nuclear transcription factor was expressed in basal cells of multilayered epithelia and myoepithelial cells of the breast. 14-3-3 was a putative tumour suppressor gene, transactivated by p53 in response to DNA damage and immunohistochemically demonstrated that 14-3-3 was consistently expressed in the cytoplasmic compartment and occasionally in the nuclei of myoepithelial ...

Dercum's disease (adiposis dolorosa) is characterised by adiposity and chronic pain in the adipose tissue. It has been proposed that conditions encompassing chronic pain have altered concentrations of neuropeptides involved in pain transmission. The aim of this investigation was to examine whether patients with Dercum's disease have abnormal concentrations of different neuropeptides. In cerebro...

Despite the strong association between epilepsy and psychiatric comorbidities, data on clinicopathologic correlations are scant. We previously reported differential mossy fiber sprouting (MFS) in mesial temporal lobe epilepsy (MTLE) patients with psychosis (MTLE + P) and major depression (MTLE + D). Because neurotrophins (NTs) can promote MFS, here, we investigated MFS, neuronal density and imm...