We describe the implementation of a Java-based application for differential diagnosis of hematopoietic neoplasms using immunophenotyping by flow cytometry. The current version of this Java applet includes the knowledge-base for 33 hematopoietic neoplasms and 43 diagnostic immunophenotyping markers. Java, a new object-oriented computing language, helps facilitate development of this applet, a pl...

Abstract Background Leukemia is one of the most common tumors in children and it is divided up into two main groups acute and chronic leukemia. The acute leukemia is more prevalent than chronic in children. Generally acute type is included acute lymphoid leukemia (ALL) and acute myeloid leukemia (AML). In this study, patients with leukemia who were admitted in Talghani hospital of Gorgan were...

Immunophenotyping is essential for lymphocytecloned classification, prognosis and treatment option in human and canine lymphomas. Immunohistochemistry (IHC) is a classic method that is referred to a gold standard due to its accuracy (Valli et al., 2011; Thalheim et al., 2013). Regarding tissue specimens, it provides morphology of lymph nodes (LN) for histological assessment, but generalized ane...

Abstract Cellular therapies such as chimeric antigen receptor (CAR) T or NK cells undergo phenotypic characterization during discovery and development of novel therapies, biomanufacturing, upstream patient cell processing for clinical use. Samples are routinely analyzed via flow cytometry cell, B monocyte surface marker expression. Image systems perform cell-based assays that can be used a conv...

Amino acid substitutions in platelet membrane glycoproteins result in alloantigens. Identifying these polymorphisms is important in alloimmune-mediated platelet disorders. Immunophenotyping platelet antigens can be limited by the unavailability of specific antisera. The goal of this work was to identify human platelet antigen genotypes in individuals using a technique that would circumvent the ...

background: despite the extensive information available in the literature, cell surface marker signature of human amniotic epithelial cells (haecs) remains controversial. the aim of the present study was to characterize immuno-phenotypic features, proliferative capacity and immunogenicity of haecs. we also tested whether expression of some cell surface markers is influenced by the type of tryps...