The synthesis and transport of the soluble antigen of influenza A virus were traced by immunofluorescent staining with human convalescent sera during abortive and complete infection of hamster BHK21 ceils and mouse L ceils. Times of synthesis in the nucleus, transport to the cytoplasm and extrusion by the cell were the same in complete and in abortive infection. The product of abortive infectio...

Introduction: The implantation is a complex procedure that involves many molecules. One of these molecules is integrin specially &alphav&beta3 which serves as receptor for components of extra cellular matrix to act as bridging molecules between the blastocyst and the endometrial surface during the implantation process. By blocking &alphav&beta3 interactions, the implantation can be impaired. ...

The synthesis and transport of the soluble antigen of influenza A virus were traced by immunofluorescent staining with human convalescent sera during abortive and complete infection of hamster BHK21 ceils and mouse L ceils. Times of synthesis in the nucleus, transport to the cytoplasm and extrusion by the cell were the same in complete and in abortive infection. The product of abortive infectio...

An antibody was detected in the sera from patients with systemic lupus erythematosus (SLE) and central nervous system (CNS) involvement that reacted with a 50-kD antigen in the plasma membrane of brain synaptic terminals. The 50-kD antigen was solubilized with Triton X-100 from preparations enriched with synaptic plasma membranes, and was partially purified by molecular sieve filtration column ...

Sera from patients with certain autoimmune diseases tht contained autoantibodies to nonhistone nuclear antigens were used as reagents in an indirect immunofluorescent study. The distribution of these nuclear antigens was determined in synchronized human B lymphoid cells. Autoantibodies to Sm antigen, nuclear ribonucleoprotein complex and SS-B antigen were used. Although all three nonhistone ant...

Isolation of rat islets of Langerhans was carried out by using collagenase and the destruction of exocrine tissue was performed through the injection of Hanks' solution into the common bile duct. The identification of the islets was done by techniques including the use of a stereomicroscope against a black background. Vital staining was done by an injection of neutral red solution through t...

Significant advances in the techniques available for rabies diagnosis have taken place over the past thirty years. The traditional method of histological examination for Negri bodies in the brain sections has been largely replaced by immunofluorescent staining methods for the detection of nucleocapsid antigen in brain smears (fluorescent antibody test: FAT) (Dean and Abelseth, 1973). Neverthele...

We previously reported that cutaneous vasculitis could be associated with anti-PS/PT antibody and LAMP-2. The copy number of LAMP-2 mRNAs in skin samples from rats induced by intravenous administration after subcutaneous histone injection was significantly higher than without vasculitis. Male Wistar-Kyoto (n=14) were given a dorsal cell-free histones then divided into four groups. Two hours lat...

Objective(s): Eupafolin, a major active component of Eupatorium perfoliatum L., has anti-inflammatory and anti-oxidant properties. Lipopolysaccharide (LPS) is responsible for myocardial depression. A line of evidences revealed that LPS induces autophagy in cardiomyocytes injury. This study aims to evaluate the effects of eupafolin on LPS-induced cardiomyocyte autophagy...

The distribution and transport in platelets of human coagulation Factor V was investigated by immunofluorescent and immunoelectron microscopy. In resting intact platelets, little surface staining was observed by immunofluorescence. In permeable resting cells, punctate staining similar to that reported for fibrinogen (Fbg), thrombospondin (TSP), fibronectin (Fn), von Willebrand factor (VWF), B-t...