نتایج جستجو برای: immunoassays
تعداد نتایج: 5308 فیلتر نتایج به سال:
Because of the global spread of Zika virus, accurate and high-throughput diagnostic immunoassays are needed. We compared the sensitivity and specificity of 5 commercially available Zika virus serologic assays to the recommended protocol of Zika virus IgM-capture ELISA and plaque-reduction neutralization tests. Most commercial immunoassays showed low sensitivity, which can be increased.
Immunoassays are widely used for determination of the concentration of steroid hormones. However, obtaining specific anti-steroid monoclonal antibodies remains difficult. We used antibody engineering and phage display methods to change the specificity of an anti-cortisol monoclonal antibody towards estradiol. This work demonstrates that production of recombinant antibodies may be a valuable way...
Silver-enhanced nanoparticle-labeled immunoassays provide a simple, low-cost, and effective way of detecting antigens in dilute solutions. The physical mechanisms behind their operation, however, have not been fully investigated. We present a semiquantitative approach for optimizing sandwich nanoparticle immunoassays using an adsorption-controlled kinetic model. Primary antibodies were immobili...
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