This review is a synopsis of different bioprocess engineering approaches adopted for the production of marine enzymes. Three major modes of operation: batch, fed-batch and continuous have been used for production of enzymes (such as protease, chitinase, agarase, peroxidase) mainly from marine bacteria and fungi on a laboratory bioreactor and pilot plant scales. Submerged, immobilized and solid-...

In this review, factors that contribute to enhancing the stability of immobilized enzyme membranes have been indicated, and the solutions to each factor, based on examples, are discussed. The factors are divided into two categories: one is dependent on the improvement of enzyme properties, and the other, on the development of supporting materials. Improvement of an enzyme itself would effective...

we expressed and purified a recombinant p. pyralis luciferase with n-terminal his-tags. the silanized ni or cu-loaded magnetic particles were prepared and used to assemble the his-tagged p. pyralis luciferase. this enzyme immobilized on functionalized magnetic nanoparticles (mnps) via electrostatic interactions of his-tag with ni2+/cu2+ ions on the surface of mnps using simple one step method. ...

Penicillin G acylase from E. coli TA1 was immobilized by Cross-Linked Enzyme Aggregates (CLEA), a new method for immobilization. This biocatalyst and commercial immobilized penicillin G acylase (PGA-450) were used to study the effect of pH, temperature and substrate concentration on the synthesis of ampicillin from phenyl glycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA). Compare...

A cantilever device based on competitive binding of an immobilized receptor to immobilized and soluble ligand and capable of measuring solution-phase thermodynamic quantities is described. Through multiple binary queries, the device stochastically measures the probability of the formation of a bound complex between immobilized protein and immobilized ligand as a function of soluble ligand conce...

OBJECTIVES (S)-Mandelate dehydrogenase (SMDH) and laccase were immobilized on chitosan. The bi-enzymatic system with immobilized SMDH and immobilized laccase was taken to catalyze the stereoselective transformation of racemic mandelic acid and (R)-mandelic acid was obtained from its racemic mixture. RESULTS Characteristics of the immobilized enzymes were valuated. The optimum pH and temperatu...

conclusions our findings clearly indicate a synergistic effect of waterpipe smoke, immobilization and darkness stress on serum ck levels, whereas serum alp levels of experimental groups have shown no significant synergism for the mentioned factors. results the results indicated enhanced serum ck and alp levels in all groups compared with control (p < 0.05). also, there was a significant differe...

Diffusion in pure gels and gels with immobilized cells was analyzed. A model of diffusion assuming a homogeneous cell distribution in gel was improved by introducing a tortuosity value. By theoretical analysis and numerical modeling it was shown that the tortuosity of a gel with immobilized cells is the product of two factors: (1) tortuosity generated by the cells, Tc, and (2) tortuosity of the...

The present study focus on the biodegradation of trinitrotoluene (TNT) by Bacillus sp. YRE1 isolated from red effluent in free state and also cells immobilized on charcoal and polystyrene. TNT degradation was monitored weekly for 168 hours, at 262 nm. Immobilized Bacillus sp. YRE1 was checked for its ability to degrade TNT by exposing it to different temperatures. It was found that both charcoa...