Constitutively active promoter elements for heterologous protein production in Lactococcus lactis are scarce. Here, the promoter of the PTS-IIC gene cluster from L. lactis NZ3900 is described. This promoter was cloned upstream of an enhanced green fluorescent protein, GFPmut3a, and transformed into L. lactis. Transformants produced up to 13.5 μg of GFPmut3a per milliliter of log phase cells. Ad...

Running Title: BRCA2 regulates cytokinesis by myosin-IIC ATPase activation

The fracture toughness of snow in shear is one of the most relevant parameters when studying the mechanics of snow slab avalanche release. Double-cantilever snow beams were loaded asymmetrically to determine K(IIc). If crack surfaces touch under applied pressure, the fracture toughness in shear of snow (rho=247 kg m(-3)) is K(IIc)=680+/-60 Pa m(1/2). Screening of the stress intensity at the cra...

Background and study aims  Visual assessment of laterally spreading tumors non-granular type (LST-NG) and depressed lesions by narrow band imaging (NBI) without magnification has not been studied. We investigated the role of non-magnifying NBI in detecting LST-NG and type IIc lesions on colonoscopy. Patients and methods  This retrospective study examined consecutive patients diagnosed as havi...

A muscle biopsy from the vastus lateralis muscle of a strength-trained woman was found to contain an unusual fiber type composition and was analyzed by histochemical, biochemical, and ultrastructural techniques. Special attention was given to the C-fibers, which comprised over 15% of the total fiber number in the biopsy. The mATPase activity of the C-fibers remained stable to varying degrees ov...

The mannitol transporter EII(mtl) from Escherichia coli is responsible for the uptake of mannitol over the inner membrane and its concomitant phosphorylation. EII(mtl) is functional as a dimer and its membrane-embedded C domain, IIC(mtl), harbors one high affinity mannitol binding site. To characterize this domain in more detail the microenvironments of thirteen residue positions were explored ...

The glucose transporter of the bacterial phosphotransferase system couples vectorial translocation to phosphorylation of the transported sugar. It consists of a transmembrane subunit (IICBGIc) and a hydrophilic subunit (IIAG1"). The IICBGIC subunit consists of two domains. The NH,-terminal IIC domain (residues 1-386) spans the membrane eight times and contains the substrate binding site. The CO...

The flameproof enclosure type of protection is still one the safest because it based on a very simple and, therefore, unlikely fallible technology. If an explosive atmosphere penetrates producing trigger, explosion occurs, but remains confined within enclosure. EN 60079-1 standard, states that flanged flame paths are allowed in presence gases and vapours Group IIB, cannot be used with IIC. Also...

Trypanosoma cruzi is thought to undergo predominant clonal evolution, as determined by population genetics studies. However, this model does not exclude occasional recombination, which existence is strongly suggested by several recent studies. We sequenced a portion of the maxicircle cytochrome b (CYb) gene and of the nuclear rRNA promoter region from representative strains of six T. cruzi gene...

Some enterotoxigenic Escherichia coli (ETEC) produce a type II heat-labile enterotoxin (LT-II) that activates adenylate cyclase in susceptible cells but is not neutralized by antisera against cholera toxin or type I heat-labile enterotoxin (LT-I). LT-I variants encoded by plasmids in ETEC from humans and pigs have amino acid sequences that are ≥ 95% identical. In contrast, LT-II toxins are chro...