background beta-thalassemia patients receive blood products from blood transfusion centers repeatedly. blood transfusion can transmit cytomegalovirus (cmv) and toxoplasma gondii. the aim of this study was serological evaluation of these two infectious agents in thalassemia patients. materials and methods in a cross-sectional study, the enzymelinked immunosorbent assay (elisa) testing was perfor...

antiphospholipid antibodies (apla) may be detected in normal pregnancies and also may cause thrombosis, recurrent fetal loss, placental infarction and preeclampsia. in the present study the possible differences in apla titer between healthy pregnant women and preeclampsia cases without history of thrombosis was examined. the apla titer in 50 healthy pregnant women with 50 preeclampsia cases wit...

A solid-phase antibody capture hemadsorption (SPACH) assay was developed to detect hepatitis A virus (HAV)-specific immunoglobulin M (IgM) antibodies in sera from humans recently infected with hepatitis. The assay is performed with microtiter plates coated with anti-human IgM antibodies to capture IgM antibodies from the test sera. HAV-specific IgM antibody is detected by the addition of HAV he...

Antibody against a cross-reactive idiotype (CRI) on human IgM-rheumatoid factor (RF) antibodies was induced by immunization of rabbits with a synthetic peptide ( PSL2 ) corresponding to the second complementarity-determining region (CDR), and adjacent amino acid residues of the kappa light chain of the IgM-RF Sie . The anti-peptide antibody bound efficiently to IgM-RF proteins known to share a ...

Solid-phase immunoglobulin M (IgM) antigen capture enzyme immunoassay (AgCEIA) and antibody capture enzyme immunoassay (AbCEIA) were developed for the diagnosis of BK virus (BKV) infections. Of 37 serum samples from renal allograft recipients, 15 were positive for BKV IgM antibody by either AgCEIA, AbCEIA, or antigen capture radioimmunoassay. False-positive IgM results were observed in the AgCE...

Two commercially available enzyme immunoassays and one assembled in house were used to measure immunoglobulin M (IgM) antibody to cytomegalovirus (CMV) in a total of 220 serum specimens from 104 renal transplant recipients. All assays included a step in which interfering IgG antibody was removed or complexed. Concordance of results between pairs of assays ranged from 84 to 96%. All sera from pa...

The C'1a-fixing properties of purified rabbit IgM anti-benzenearsonate antibody were determined. When tested with sheep erythrocytes to which hapten had been coupled by diazo linkage, the number of C'1a molecules fixed was 21% of the number of IgM antibody molecules bound to the erythrocyte surface. This was not due to loss of C'1a-fixing capacity during the purification procedure. Preparative ...

Study objective — assessment of the humoral and cell-mediated immunity features in COVID-19 convalescents three months after their discharge from hospital. Material Methods The study involved 78 who, depending on profile specific IgM IgG antibodies to SARS-CoV-2, were divided into groups. control group consisted 50 volunteers. Detection blood serum was performed by ELISA. Determination CRP conc...