A laboratory study was carried out on 44 sera of typhoid patients (age: 15-45 years, 24 females and 20 males) with positive blood culture for S. typhi, 41 sera non typhoid febrile patients (age: 16-51 years, 17 females & 24 males) with negative blood culture and negative Widal test, 136 sera of healthy persons as normal controls (age: 20-49 years, 95 females & 41 males). The basic principle of ...

BACKGROUND With society's rapidly increasing mobility, patients infected with severe viral infections can become seriously ill at any place in Europe and elsewhere. Improving the diagnostics of these infections is the most important step in detecting the pathogens and dealing with them, and for this purpose, quality control measures are essential tools. OBJECTIVES To assess the diagnostic rea...

Congenital toxoplasmosis is associated with important morbidity and mortality. Since vertical transmission of Toxoplasma gondii can occur in acute cases, antenatal screening for recent infections is vital. Accurate determination of acute toxoplasmosis requires a combination of immunoassays, usually not routinely applied for screening purposes. This study evaluated the anti-T. gondii (IgG+IgM)/I...

Background: Helicobacter pylori (H. pylori), is a common infection in pregnant women accompanied by variations in the levels of the IgM, IgA and IgG antibody isotypes. The variations of anti-H. pylori antibodies during and after pregnancy, and the extent of protection they provide to the mother and the fetus are not completely understood. Objectives: To investigate the changes of the anti-H. py...

Gestational Toxoplasma gondii infection is considered a major risk factor for miscarriage, prematurity and low birth weight in animals. However, studies focusing on this topic in humans are scarce. The objective of this study is to determine whether anti-Toxoplasma gondii maternal serum profiles correlate prematurity and low birth weight in humans. The study examined 213 pregnant women seen at ...

To evaluate the value of IgG avidity in diagnosis of congenital cytomegalovirus (CMV) infection in newborns and infants we collected serum samples from 40 infants under 12 months of age with suspected congenital CMV infection. Sera were tested for IgM, IgG and IgG avidity. For 25 of them, virus isolation and/or polymerase chain reaction (PCR) on urine specimens were performed. Thirteen (32.5%) ...

Tumour populations from two B cell neoplasms were investigated for their capacity to synthesise immunoglobulin in vitro. Neoplastic cells from a patient with chronic lymphocytic leukaemia and an IgG lambda paraproteinaemia co-expressed surface IgG lambda and IgM lambda and synthesised both IgG lambda and IgM lambda in short-term culture. In the second patient with non-Hodgkin's lymphoma, the ne...

BACKGROUND A major drawback of modern society's rapidly increasing mobility is the ease with which dangerous infections can be imported into Europe. Often these infections are not diagnosed because physicians are not familiar with the symptoms and laboratory tests are not always available in local diagnostic centres. Improving diagnostics is the most important step in detecting and dealing with...

Tumour populations from two B cell neoplasms were investigated for their capacity to synthesise immunoglobulin in vitro. Neoplastic cells from a patient with chronic lymphocytic leukaemia and an IgG A paraproteinaemia co-expressed surface IgG A and IgM A and synthesised both IgG A and IgM A in short-term culture. In the second patient with non-Hodgkin's lymphoma, the neoplastic cells expressed ...

The autoimmune disease antiphospholipid syndrome (APS) is characterized by the presence of anticardiolipin antibodies (aCL), along with anti-β2-glycoprotein I (β2GPI) antibodies and lupus anticoagulant (LA). In this study, we developed a time-resolved fluoroimmunoassay (TRFIA) system for simultaneous quantification of aCL IgG and IgM. A 96-well microtiter plate precoated with the complex of car...