BACKGROUND AND OBJECTIVES Brucellosis is one of the most common zoonotic diseases in Iran and human brucellosis is endemic in all parts of the country. Because of the difficulty in the diagnosis of brucellosis, particularly in endemic areas, the use of new and feasible diagnostic tests seem to be of great importance for resolving the diagnostic obstacles. We evaluated the usefulness of a new se...

BACKGROUND It has been suggested that the presence of Toxocara canis larvae in lungs is an underlying factor in the onset of asthma. Although the association of asthma and seropositivity to Toxocara has been observed, there are no studies that indicate whether these antibodies are specific against T. canis antigens. METHODS Seroprevalence to T. canis excretion-secretion antigens (TcES Ag) wer...

conclusions according to the results, brucellosis has remained endemic in this region. most cases were detected by elisa igg. the highest kappa agreements were between tests krb-sat, krb-igg and ksat-igg, while the lowest levels of agreement were between tests sat-igm and elisa igm-igg. considering that elisa igm results are covered by sat and elisa igg test results, applications of this test d...

The high avidity (HA) anti-dsDNA IgG ELISA is considered highly specific for the diagnosis of systemic lupus erythematosus (SLE). The main objective of this study was to determine the performance of this test with existing assays for detecting anti-dsDNA IgG antibodies as well as assess its analytical characteristics. For method comparison studies, we investigated the correlation between the HA...

A new commercial anti-Japanese encephalitis virus IgM and IgG indirect immunofluorescence test (IIFT) was evaluated for the detection of the humoral immune response after Japanese encephalitis vaccination. The IgM IIFT was compared to two IgM capture ELISAs and the IgG IIFT was analysed in comparison to a plaque reduction neutralization test (PRNT50) and an IgG ELISA. Moreover, the course of th...

A new commercially available rapid screening immunochromatographic test (ICT) for Detection of anti-rubella IgG & IgM antibodies was compared with ELISA. Materials and methods: 161 serum samples were tested by immunochromatography card test (Rubella IgG/IgM, SD –Bioline) and were compared with a standard IgG & IgM ELISA (Ani Biotech Oy, Orgenium Laboratories, Vantaa, Finland). Results: ELISA an...

Since its introduction in 1999, West Nile virus (WNV) infections have spread rapidly across the North American continent. Diagnosis of acute WNV infection by detection of WNV-specific immunoglobulin M (IgM) is complicated by the persistence of detectable IgM for more than 1 year in some patients. IgG antibody avidity testing was assessed as a supplemental assay in the diagnosis of current infec...

Toxoplasma gondii is an obligate intracellular protozoan that is distributed worldwide. Recently, several tests for avidity of Toxoplasma IgG antibodies have been introduced to help discriminate between recently acquired and distant infections. The study was conducted in Jawaharlal Nehru Medical College and Hospital, India from February 2011 to September 2012. Serum specimens were subjected to ...

Background: Brucellosis is a world-wide disease, which has a diverse clinical manifestation, and its diagnosis has to be proven by laboratory data. Serum agglutination test (SAT) is the most-widely used test for diagnosing brucellosis. The enzyme linked immunosorbent assay (ELISA) can also determine specific antibody classes against brucella. It is a sensitive, simple and rapid test, which coul...