Rift Valley fever virus (RVFV) is an important human and animal pathogen in Africa and has been responsible for infections in travelers. Because of the aerosol infectivity and risk of dissemination of the virus, a need exists for simple, safe, serological tests for diagnosis. An enzyme-linked immunosorbent assay (ELISA) was developed to detect RVFV-specific immunoglobulins (immunoglobulin G [Ig...

different igg subclass profiles are produced in response to different antigenic stimuli in a variety of diseases. igg subclass levels may reflect disease severity. quantification of igg subclasses depends on the availability of specific monoclonal antibodies (mabs). in the present study seven hybridoma clones producing mabs reactive with multiple subclasses of human igg were established. spleno...

background: toxoplasma specific igm antibodies; the common serologic marker in diagnosis of acute toxoplasmosis has its own limitations. confirmatory testing with other markers, introduced as a complementary tool in distinguish acute and chronic infections is unusual in iran. in the present study, we investigated the cor-relation between the results of igm elisa, iga elisa, and igg avidity test...

background: the diagnosis of toxocariasis heavily depends on immunological tests because the number of parasites is usually few in infected tissues, unless they migrate into an organ such as eye. in general, patients with ocular toxocariasis have serum anti- t canis antibody titres that are significantly lower than those with visceral toxocariasis. objective: to diagnose the asymptomatic toxoca...

BACKGROUND Detection of Trypanosoma cruzi antigens in clinical samples is considered an important diagnostic tool for Chagas disease. The production and use of polyclonal antibodies may contribute to an increase in the sensitivity of immunodiagnosis of Chagas disease. METHODOLOGY/PRINCIPAL FINDINGS Polyclonal antibodies were raised in alpacas, rabbits, and hens immunized with trypomastigote e...

Recently it has been reported, that immunoglobulin Y (IgY) can be used instead of polyclonal antibodies extracted from mammals (IgG) for the purpose of diagnosis and therapy. These antibodies are found to have better properties in terms of specificity and ease of large-scale production. In addition, IgY binds neither to mammalian complement or Fc-receptors nor does it interfere with rheumatoid ...

Recruitment of natural antibodies towards tumour cells for their elimination by the immune system could be a highly specific and efficacious therapeutic strategy. While l-rhamnose has already been explored as suitable antigen antibody recruitment, we here report first rhamnose-based glycomimetic to used such purpose. The is designed more hydrolytically enzymatically stable than rhamnosides, pro...

background: helicobacter pylori (hp) is a gram negative, spiral shaped bacterium which colonizes the gastric mucosa and induces gastroduodenal complications varying from mild gastritis with no clinical complications to peptic ulcer diseases and even gastric malignancies. the rate of hp infection is 30-50% in developed countries whereas it has been rated up to 80% of the adult population in deve...

conclusion it was concluded that elisa-based anti h. pylori igg test in saliva could be used as an alternative diagnostic test in the absence of other invasive procedures. background helicobacter pylori is known as an agent which may involve in the occurrence of peptic ulcer, gastric cancer and also other known and unknown diseases. treatment of the infection with antibiotics eradicates the dis...