Defects in the biosynthesis of N- and core 1 O-glycans may be found by isoelectric focusing (IEF) of plasma transferrin and apolipoprotein C-III (apoC-III). We hypothesized that IEF of transferrin and apoC-III in combination with sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of apoC-III may provide a classification for congenital disorders of glycosylation (CDG) patients...

The objective of this study was to determine the responses in the digestibility of dry matter (DM) and amino acid (AA) composition of ileal endogenous flow (IEF) of pigs (initial body weight, 69.1±6.46 kg) fed N-free diets (NFD) formulated with different ratios of corn starch to dextrose. Fifteen pigs fitted with a T-cannula at the distal ileum were fed 5 diets according to a triplicated 5×2 in...

The method of 2‑dimensional gel electrophoresis (2-DE) has been widely used for the proteomic profiling of solid biological samples, however, the analytical conditions have not been optimized. The present study optimized the major conditions of 2‑DE for determining the protein contents of solid tumor tissues, through enhancement of the separation efficiency and resolution. Three major analytica...

[1] Using 8 years of ionospheric drift measurements from the low-latitude JULIA (Jicamarca Unattended Long-term Investigations of the Ionosphere and Atmosphere) radar and the solar wind and interplanetary magnetic field data from the ACE (Advance Composition Explorer) satellite, we study the characteristics of the prompt penetration of electric fields to the equatorial ionosphere. A large datab...

A poly(dimethylsiloxane)(PDMS)/SU-8/quartz hybrid chip was developed and applied in the isoelectric focusing (IEF) of proteins with ultraviolet (UV) absorbance-based whole-channel imaging detection (UV-WCID). Each hybrid chip was made of three layers: a PDMS flat top substrate, a bottom quartz substrate and a middle layer of SU-8 photoresist. The SU-8 serves two purposes: it contains the microc...

for screening for Hb variants, such as electrophoresis, IEF, or ion-exchange HPLC, are not sufficient because 33 of the 89 different Hb variants associated with erythrocytosis do not induce any major change in net charge of the Hb molecule. The additional use of ESMS is encouraged because combination of this technique and IEF will increase the sensitivity for Hb variants that cause erythrocytos...

BACKGROUND The diagnostic accuracy of cerebrospinal fluid oligoclonal bands (CSF-OCB) detected by isoelectric focusing (IEF) in patients with multiple sclerosis (MS) was evaluated in our study. METHODS Three hundred and twenty-one patients with MS and other central nervous system (CNS) immune mediated disorders were assessed (CIMD). Cerebrospinal fluid and matched serum samples were examined ...

(DNJ) in Peru exemplifies how one small eye clinic can become sustainable, grow, and influence national eye care strategies. DNJ was established in 1996 and initially provided general health care. From 2006 onwards, CBM Latin America helped DNJ to focus on eye care and later sponsored a DNJ leadership team to attend an International Eye Foundation (IEF) sustainability workshop in Paraguay. DNJ ...

OBJECTIVES To investigate the utility of isoelectric focusing electrophoresis (IEF) for identifying patients with α-thalassemia, which results from the deletion of 1 or more of the α-globin genes. METHODS Samples were selected based on their hemoglobin H (HbH) concentration observed using IEF. The samples were analyzed for the most common α-globin gene deletions using molecular analysis. RE...