We examined the impact of hypoxia-ischemia on high-affinity [3H]glutamate uptake into a synaptosomal fraction prepared from immature rat corpus striatum. In 7-day-old pups the right carotid artery was ligated, and pups were exposed to 8% oxygen for 0, 0.5, 1, or 2.5 h, and allowed to recover for up to 24 h before they were killed. High-affinity glutamate uptakes in striatal synaptosomes derived...

Hypoxia to the tissue plays a significant role in ischemia. In spite of the development of various in vitro models of neuronal cultures to study the mechanism of ischemia-induced cell death, not much is known about the role of protein expression in ischemia induced pheochromocytoma (PC12) cells. Present study was done to study the expressions of proteins in hypoxia induced PC12 cells and after ...

Lactate is an intriguing molecule with emerging physiological roles in the brain. It has beneficial effects animal models of acute brain injuries and traumatic injury or subarachnoid hemorrhage patients. However, mechanism by which lactate provides protection unclear. While there evidence a metabolic effect providing energy to deprived neurons, it can also activate hydroxycarboxylic acid recept...

Background: Creatine kinase MB (CK-MB), which exists mainly in the cytoplasm of myocardial cells, is currently accepted as an indicator and has high sensitivity specificity for diagnosis injury. Objective: This study was aimed to estimate serum creatine (CK-MB) levels after asphyxia-induced injury neonates. Patients methods: 40 neonates were included this divided into 20 cases with perinatal h...

Hypoxia frequently accompanies such vascular disorders as atherosclerosis, thrombosis and ischemia/reperfusion injury. Myocardial ischemia/reperfusion, in particular, is a major contributor to cardiomyocyte impairment. Autophagy is a dynamic, self-catabolic process that has been implicated in a wide range of physiological processes and the pathogenesis of diverse diseases. The aim of the presen...

NMDARs (N-methyl-D-aspartate receptors) mediate the predominantly excitatory neurotransmission in the CNS (central nervous system). Excessive release of glutamate and overactivation of NMDARs during brain ischemia and the hypoxia process are causally linked to excitotoxicity and neuronal damage. GluN3 subunits, the third member of the NMDAR family with two isoforms, GluN3A and GluN3B, have been...

BACKGROUND Exposure to hypoxia and isoflurane (Iso) before hypoxia-ischemia has been found to be neuroprotective in neonatal rats. We investigated the long-term effects of delayed preconditioning with Iso, hypoxia, or room air on motor and cognitive function in mice that had 65 min of hypoxia-ischemia on postnatal day 10. METHODS Nine-day-old C57x129T2 F1 mice received either 1.8% Iso, hypoxi...

Plin5 is abundantly expressed in the heart where it binds to lipid droplets (LDs) and facilitates physical interaction between LDs mitochondria. We isolated cardiomyocytes from adult Plin5+/+ Plin5-/- mice study role of for fatty acid uptake, LD accumulation, oxidation, tolerance hypoxia. Cardiomyocytes cultured with oleic stored less than Plin5+/+, but comparable levels when adipose triglyceri...

The protective effects of ilexsaponin A on ischemia-reperfusion-induced myocardial injury were investigated. Myocardial ischemia/reperfusion model was established in male Sprague-Dawley rats. Myocardial injury was evaluated by TTC staining and myocardial marker enzyme leakage. The in vitro protective potential of Ilexsaponin A was assessed on hypoxia/reoxygenation cellular model in neonatal rat...