Origanum vulgare contains high levels of phenolic compounds such as gallic acid and polyphenols such as rosmarinic acid and quercetin. The purpose of this study was to investigate the effects of Origanum vulgare extract, as a natural antioxidant, on freezing-thawing semen quality in Holstein bulls. Three Holstein bulls (5-6 years old, mean live weight 800 kg) were used for semen collection twic...

introduction: varicocele is considered as a common cause of male infertility. the aim of this study was to evaluate the effects of varicocelectomy on sperm parameters, sperm membrane integrity and sperm nuclear maturity. materials and methods: in this study along with routine semen analysis, hypo-osmotic swelling test (host), and aniline blue staining for assessment of as 45 and 90 days after i...

the objective of this study was to investigate aspartate-amino-transferase (ast), γ-glutamyl-transferase(ggt), alkaline phosphatase (alp), lactate-dehydrogenase (ldh) and acid phosphatase (acp) activities andsemen parameters (volume, ph, concentration, total sperm number (tsn), progressive motility, dead sperm,total morphological defect (tmd) and hypo-osmotic swelling test (host)) in seminal pl...

Hypo-osmotic swelling of human Intestine 407 cells leads to a significant increase of intracellular MAPKAP-kinase 2 activity and Hsp27 phosphorylation. Pre-treatment of the cells with the p38 MAP kinase inhibitor SB-203580 blocks this activation, indicating that the hypotonicity-induced activation of MAPKAP kinase 2 is, similarly to that described for hyperosmotic treatment, the result of an ac...

In mammalian liver, proteolysis is regulated by the cellular hydration state in a microtubule- and p38(MAPK) (p38 mitogen-activated protein kinase)-dependent fashion. Osmosensing in liver cells towards proteolysis is achieved by activation of integrin receptors. The yeast orthologue of p38(MAPK) is Hog1 (high-osmolarity glycerol 1), which is involved in the hyperosmotic-response pathway. Since ...

Antibiotics are added to semen extenders to take care of heavy microbial load, however, their continuous use poses a constant threat of developing antibiotic resistance by the common microbes present in the semen. Our hypothesis was that natural honey, having antibacterial activity and rich in fructose could replace the use of antibiotics and fructose in the semen extender. Twenty-four ejaculat...

This study aimed at determining the physico-morphological attributes, in vitrofertilityand their interrelationships in semen of Sahiwal bulls. Semen samples werecollected weekly for 6 weeks from ten mature Sahiwal bulls divided randomly into 5 groups. The mean (±SEM) of ejaculate volume (mL), mass activity (score 0-5), progressive sperm motility (%), sperm concentration (106/mL), live sperm (%)...

Conventional semen analysis solely is not completely adequate to predict pregnancy outcomes. Therefore, advanced sperm function tests have been developed and introduced to clinical practice. These tests use different methods and techniques to evaluate different stages of fertilization steps. In this review, we reported some commonly used sperm function tests: sperm penetration assay, sperm-zona...