نتایج جستجو برای: hydroxybutyrate phb

تعداد نتایج: 4992  

2017
Mariana Itzel Velasco Alvarez Angela ten Pierick Patricia T. N. van Dam Reza Maleki Seifar Mark C. M. van Loosdrecht S. Aljoscha Wahl

Poly(3-hydroxybutyrate) (PHB) is an interesting biopolymer for replacing petroleum-based plastics, its biological production is performed in natural and engineered microorganisms. Current metabolic engineering approaches rely on high-throughput strain construction and screening. Analytical procedures have to be compatible with the small scale and speed of these approaches. Here, we present a me...

2015
Vaishnavi Gowda U. S. Srividya Shivakumar

Very few studies have been dedicated to R-hydroxyacids (R-HA) production using extracellular polyhydroxyalkanoate depolymerases (ePhaZs). Penicillium expansum produced maximum extracellular polyhydroxybutyrate depolymerase (~6 U/mL) by 72 h when grown in mineral salt medium containing 0.2 % w/v PHB, pH 5.0, at 30 °C and 200 rpm shaking conditions. Partial purification of the extracellular poly(...

Journal: :Biomacromolecules 2005
Adam G Lawrence Joonho Choi Chokyun Rha Joanne Stubbe Anthony J Sinskey

Allochromatium vinosum polyhydroxyalkanoate synthase catalyzes formation of poly-(R)-3-hydroxybutyrate (PHB) from (R)-3-hydroxybutyryl-coenzyme A (HB-CoA). (R)-3-Hydroxybutyryl-N-acetylcysteamine (HB-NAC) is an alternative substrate for this synthase in vitro, with a turnover 1% that of HB-CoA. With HB-NAC, the molecular weight (M(w)) of PHB produced at substrate-to-enzyme ratios of 1500-15 000...

2012
Lakshmi Tripathi Lin-Ping Wu Jinchun Chen Guo-Qiang Chen

BACKGROUND Block polyhydroxyalkanoates (PHA) were reported to be resistant against polymer aging that negatively affects polymer properties. Recently, more and more attempts have been directed to make PHA block copolymers. Diblock copolymers PHB-b-PHHx consisting of poly-3-hydroxybutyrate (PHB) block covalently bonded with poly-3-hydroxyhexanoate (PHHx) block were for the first time produced su...

Journal: :iranian journal of chemistry and chemical engineering (ijcce) 2009
zahra b. mokhtari-hosseini ebrahim vasheghani farahani sayed abbas shojaosadati ramin karimzadeh kianoush khosravi darani

plackett-burman design was used for selection of important media components such as carbon and nitrogen sources and minerals which affect poly(hydroxybutyrate) production and cell growth of methylobacterium extorquens dsmz 1340. among the studied variables, nitrogen and phosphorus sources, mgso4 and most of the trace elements were found to be significant variables for phb production from methan...

Journal: :Bioscience, biotechnology, and biochemistry 2002
Yoshikatsu Suzuki Minoru Kurano Yuko Arai Hideo Nakashita Yoshiharu Doi Ron Usami Kohki Horikoshi Isamu Yamaguchi

Chemical regulation of secondary-metabolite synthesis was investigated through the improvement of poly-3-hydroxybutyrate (PHB) production in transgenic tobacco plants by the use of enzyme inhibitors. Two tobacco lines, BC3 and rCAB8, that produce PHB in both the cytosol and plastids were used. An acetyl-CoA carboxylase inhibitor, D-(+)-Quizalofop-ethyl, increased PHB accumulation in both lines ...

Journal: :Applied and environmental microbiology 2000
J Sun X Peng J Van Impe J Vanderleyden

Azospirillum brasilense Sp7 and its ntrA (rpoN), ntrBC, and ntrC mutants have been evaluated for their capabilities of poly-3-hydroxybutyrate (PHB) accumulation in media with high and low ammonia concentrations. It was observed that the ntrBC and ntrC mutants can produce PHB in both low- and high-C/N-ratio media, while no significant PHB production was observed for the wild type or the ntrA mut...

Journal: :Bioprocess and biosystems engineering 2014
Abeed Fatima Mohidin Batcha D M Reddy Prasad Maksudur R Khan Hamidah Abdullah

Poly(3-hydroxybutyrate) (PHB) is a biodegradable polymer that can be synthesized through bacterial fermentation. In this study, Cupriavidus necator H16 is used to synthesize PHB by using Jatropha oil as its sole carbon source. Different variables mainly jatropha oil and urea concentrations, and agitation rate were investigated to determine the optimum condition for microbial fermentation in bat...

Journal: :jundishapur journal of microbiology 0
maryam jari genetics department, faculty of science, shahid chamran university of ahvaz, ahvaz, ir iran saeid reza khatami genetics department, faculty of science, shahid chamran university of ahvaz, ahvaz, ir iran; genetics department, faculty of science, shahid chamran university of ahvaz, ahvaz, ir iran. tel: +98-6133338965 hamid galehdari genetics department, faculty of science, shahid chamran university of ahvaz, ahvaz, ir iran mohammad shafiei genetics department, faculty of science, shahid chamran university of ahvaz, ahvaz, ir iran

background poly 3-hydroxybutyrate (phb), a class of poly hydroxyalkanoates (phas), is a group of bacterial storage polymers, produced by various microorganisms in response to nutrient limitation. phas are biodegradable polymers which could be a good substitute for current petrochemical plastics. phb has been synthesized during three enzymatic steps including three genes. objectives our aim was ...

1998
Fang Liu Wenqing Li Darin Ridgway Tingyue Gu Zhongyao Shen

Beet molasses successfully replaced glucose as sole carbon source to produce poly-b-hydroxybutyrate by a recombinant Escherichia coli strain (HMS174/pTZ18u-PHB). The fermentation with molasses was cheaper than with glucose. The final dry cell weight, PHB content and PHB productivity were 39.5 g/L, 80% (w/w) and 1 g/Lh, respectively, in a 5 L stirred tank fermenter after 31.5 h fed-batch ferment...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید