ZZ polyester beads are polyhydroxyalkanoate granules which display, at high density, the ZZ domain of protein A as a fusion protein covalently linked to the polyester core. These granules are produced directly, in one step, by recombinant E. coli. We have used these granules produced in a one step process to purify mouse IgG1, IgG2a and IgG2b from mouse hybridoma supernatants. Purified immunogl...

Monoclonal B cell hybridoma cell lines express glycolipids characteristic of both the myeloma and normal lymphocyte parents. The neutral glycolipids from hybridoma cell lines metabolically radiolabeled with [14C]galactose plus [14C]glucosamine separate by high performance thin layer chromatography into patterns that may reflect differences in glycolipid expression among B cell subsets. Gas chro...

A hybridoma antibody (11E7-1) was isolated from a myeloma fusion with nu/nu BALB/c immunized against the T15 idiotype. This IgM antibody exhibited a dual specificity, binding both to PC and to anti-PC antibodies from two idiotype families. Binding to PC and anti-PC antibodies are completely inhibited by PC analogs. Furthermore, the hybridoma antibody binds to itself. Self-binding is also inhibi...

Like Ig genes, TCR genes are formed by somatic rearrangements of noncontiguous genomic V, J, and C regions. Unlike Ig genes, somatic hypermutation of TCR V regions is an infrequent event. We describe the occurrence of spontaneous hypermutation in a nonproductively rearranged TCR alpha-chain gene in a clonal T cell hybridoma that had lost its productively rearranged alpha-chain. The mutating hyb...

A major advantage of using monoclonal antibodies over polyclonal antisera is the potential availability of large quantities of the specific monoclonal antibody. In general, preparations containing the monoclonal antibody include a hybridoma supernatant, ascites fluid from a mouse inoculated with the hybridoma, and purified monoclonal antibody. Hybridoma supernatants are easy to produce, especia...

Hybridomas are the specific cells that have specific character to produce desire antibody. To produce monoclonal antibodies, β cells are removed from the spleen of an animal that has been immunized with the releant antigen. These β cells are then fused with myeloma tumor cells in the presence of PEG. The use of HGPRT cells assured that only hybridomas are selected. The fused hybrid cells (hybri...

During the past several years, our laboratory has studied the mechanism of immune suppression in the 4-hydroxy-3-nitrophenyl acetyl (NP) 1 system. In this model system, both cell-mediated and humoral immunity (1-3) have been analyzed. The findings have permitted integration of the data into a single linear scheme of cellular interactions (3-5). Based on the results obtained in our and other lab...

Monoclonal antibodies can specifically bind or even inhibit drug targets and have hence become the fastest growing class of human therapeutics. Although they can be screened for binding affinities at very high throughput using systems such as phage display, screening for functional properties (e.g., the inhibition of a drug target) is much more challenging. Typically these screens require the g...