Fusion of clonal neuroblastoma cells with rat glioma cells yielded clonal hybrid cell lines which synthesize, store and excrete acetylcholine; properties which are not expressed by the parental cell lines. Cells from one hybrid line were found to form synapses with cultured striated muscle cells. Synapses between hybrid cells ar&muscle cells closely resemble the synapses between normal motor ne...

INTRODUCTION We have presented a substantial body of experiment to show that when cells from a range of different highly malignant mouse tumours are fused with cells of low tumorigenicity, the malignant phenotype is suppressed in the hybrid cell (Harris, Miller, Klein, Worst & Tachibana, 1969; Klein, Bregula, Wiener & Harris, 1971; Harris, 1971). The most decisive results were obtained with hyb...

A solution filling and drying method has been demonstrated to fabricate Si/PEDOT:PSS core/shell nanowire arrays for hybrid solar cells. The hybrid core/shell nanowire arrays show excellent broadband anti-reflection, and resulting hybrid solar cells absorb about 88% of AM 1.5G photons in the 300–1100 nm range. The power conversion efficiency (PCE) of the hybrid solar cell reaches 6.35%, and is p...

We have investigated the DNase I hypersensitivity of a hybrid mouse/human beta-globin gene in erythroid and non-erythroid cells of transgenic mice, to examine the relationship between the chromatin structure and the expression of an exogenous gene. The hybrid globin gene was previously shown to be expressed specifically in erythroid cells in some transgenic lines. The maximal level of hybrid gl...

HYBRIDS FROM A CROSS OF RAT HEPATOMA CELLS WITH DIPLOID EPITHELIAL CELLS FROM RAT LIVER HAVE BEEN STUDIED WITH RESPECT TO KARYOTYPE AND EXPRESSION OF TWO FUNCTIONS LIMITED TO THE HEPATOMA PARENT: high level of the enzyme tyrosine aminotransferase (EC 2.6.1.5; L-tyrosine:2-oxoglutarate aminotransferase) and its inducibility with steroid hormones. The hybrids that contain the complete chromosomal...

An artificial three-dimensional (3D) culture system that mimics the tumor microenvironment in vitro is an essential tool for investigating the cross-talk between immune and cancer cells in tumors. In this study, we developed a 3D culture system using an electrospun poly(ε-caprolactone) (PCL) nanofibrous scaffold (NFS). A hybrid NFS containing an uninterrupted network of nano- and submicron-scal...

Humoral immune responses are known to be regulated, both positively and negatively, by antigenand idiotype-specific T lymphocytes. Despite the large body of evidence supporting this notion (1-3), the mechanisms by which T cells influence B lymphocyte differentiation and function are largely unknown. One of the major reasons for this is the vast heterogeneity of the immune system, so that only v...

Thymidine kinase-deficient OTT6050 mouse teratocarcinoma cells were fused with hypoxanthine phosphoribosyltransferase-deficient Fu5AH rat hepatoma cells by means of inactivated Sendai virus. The resulting hybrid cells, which were selected in hypoxanthine/aminopterin/thymidine medium, retained almost all of the mouse chromosomes and various numbers of rat chromosomes, and showed many chromosomal...

We demonstrate that semiconductor nanorods can be used to fabricate readily processed and efficient hybrid solar cells together with polymers. By controlling nanorod length, we can change the distance on which electrons are transported directly through the thin film device. Tuning the band gap by altering the nanorod radius enabled us to optimize the overlap between the absorption spectrum of t...

The ability of hybrid tumor cells to induce antitumor immunity has been evaluated in the line 1 alveolar cell carcinoma (L1) model of BALB/c mice. Hybrid tumor cells were produced by fusing freshly dissociated L1 cells isolated from in vivo tumors with the hypoxanthine:aminopterin:thymidine-sensitive cell line, GM 347, derived from C3H mice. Each hybrid was characterized by DNA content and expr...