background and aims. the aim of this study was to compare the biocompatibility of calcium-enriched mixture ce-ment (cem), composite resin and nano-particled mineral trioxide aggregate (np-mta) using human gingival fibroblasts. materials and methods. a comparative in vitro cell culture study was carried out using 60 single-rooted teeth which were assigned to the following four groups: 1) untreat...

Programmed cell death (apoptosis) is an active process by which cells initiate their own self-destruction. Growing evidence shows that this event is controlled by the activation of unique gene expression; some function as survival genes, such as bcl2, and others as killer genes, such as ced3 or interleukin converting enzyme. Likewise, external factors, such as the presence or absence of stimuli...

Cigarette smoking can lead to chronic lung inflammation and lung cancer. Chronic inflammation, associated with expression of cyclooxygenase-2 (COX-2) and prostaglandins, predisposes to malignancy. We recently demonstrated that human lung fibroblasts are activated by cigarette smoke to express COX-2 and prostaglandin E(2) (PGE(2)). Little is known about the mechanism whereby smoke activates huma...

Rat fibroblasts degraded human low density lipoprotein (LDL) very slowly, one-tenth to one-fortieth the rates observed in human fibroblasts. In rat cells, human LDL caused only very small increases in cell cholesterol content and acylCoA:cholesterol acyltransferase (ACAT) activity and caused only small decreases in beta-hydroxy-beta-methylglutaryl CoA (HMG CoA) reductase activity; in human cell...

this study aimed to evaluate the cellular toxicity of two newly-released acrylic resins (futura gen and gc reline hard) in comparison with the conventional heat-cure resin (meliodent).sample discs from each acrylic resin were placed in 24-well culture plates along with l929 mouse fibroblast cell line. a mixture of the rpmi 1640 medium, antibiotics and 10% fbs was added to the plates and the spe...

conclusions trichloroacetic acid and autologous fibroblast can be considered as therapeutic choice for recalcitrant diabetic foot ulcer. introduction foot ulcer, with a prevalence of 15% in general population, is one of the main complications in patients with diabetes. so far, different therapeutic methods have been provided for the treatment of foot ulcers. when no response to the standard tre...

collagens are the most abundant proteins in the human body. their main function is to provide structural and mechanical support for the tissues, but they are also involved in a number of other biological functions including cell attachment, migration and differentiation. collagens and gelatins are widely used in pharmaceutical and medical applications. every year, more than 50,000 tons of colla...