Skin dryness and thickening are hallmarks of systemic sclerosis (SSc) disease. Aquaporins (AQPs) are plasma membrane proteins that transport glycerol and water, resulting in water retention and skin hydration. Expression of AQPs has been evaluated in human normal skin. However, expression of these proteins in SSc dermal fibroblasts has not yet been reported. The aim of this study was to assess ...

normal 0 false false false en-us x-none ar-sa microsoftinternetexplorer4 one of the prominent types of connective tissue cells is fibroblast that synthesizes and maintains the extracellular matrix of many animal tissues. previous studies illustrated that calprotectin protein has different cytotoxicity effects on fibroblast cells. calprotectin is abundant in the neutrophil cytosol; it has growth...

The regulation of adhesion and the subsequent behavior fibroblast cells on surface biomaterials is important for successful tissue regeneration wound healing by implanted biomaterials. We have synthesized poly(ω-methoxyalkyl acrylate)s (PMCxAs; x indicates number methylene carbons between ester ethyl oxygen), with a carbon chain length = 2–6, to investigate cell including adhesion, proliferatio...

Dermal fibroblasts have been used clinically to promote wound healing and reduce wrinkles. Most of the time, fetal bovine serum (FBS) is for expansion fibroblasts. In addition, chemically defined medium can also be fibroblast expansion. Nonetheless, both FBS are not ideal culture cells that will as has risk pathogen transmission induction xenogeneic immune response whilst extremely expensive. t...

Dermal fibroblasts are a dynamic and diverse population of cells whose functions in skin in many respects remain unknown. Normal adult human skin contains at least three distinct subpopulations of fibroblasts, which occupy unique niches in the dermis. Fibroblasts from each of these niches exhibit distinctive differences when cultured separately. Specific differences in fibroblast physiology are...

In stem cell cultures from adult human tissue, undesirable contamination with fibroblasts is frequently present. The presence of fibroblasts obscures the actual number of stem cells and may result in extracellular matrix production after transplantation. Identification of fibroblasts is difficult because of the lack of specific fibroblast markers. In our laboratory, we isolate and expand neural...

While vasculature plays an essential role in skin physiology its architecture and function are altered aged diseased skin. There is thus a need to develop innovative 3D vitro models with adjustable study the regulation of angiogenesis. Hence, this work aims vascularized model characteristics response angiogenic stimuli. To do so, scaffold-free approach was used: primary fibroblast cell sheets w...

The multifunctional cytokine p43 acts on endothelial and immune cells to control angiogenesis and inflammation. In this report, we describe an additional activity of p43 that specifically promotes fibroblast proliferation and wound repair. In skin wound regions from mice, tumor necrosis factor-alpha induced p43 expression and secretion from macrophages recruited to the site. p43 also promoted f...

Decellularized extracellular matrix components isolated from animal sources have shown much clinical promise in the treatment of several wound care indications including a variety of dermal ulcerations (diabetic, pressure, venous, e.g.), full-thickness trauma wounds affecting the skin and skeletal muscle, and post-operative surgical wounds. The purpose of this study was to evaluate the biocompa...