Collagenases produced by human polymorphonuclear leukocytes, human lung fibroblasts, and rabbit pulmonary alveolar macrophages were compared in their ability to digest soluble native type I and type III collagens. While the fibroblast and macrophage collagenases attacked the two substrates at approximately equal rates, the leukocyte collagenase attacked type I collagen preferentially (15:1) in ...

Bone tissue is a hierarchical material characterized at nanoscale by the mineralized collagen fibril, recurring structure mainly composed of apatite minerals, and water. nanostructure has fundamental role in determining mechanical behavior its mass transport properties. Diffusion phenomenon allows to maintain an adequate supply metabolites mechanisms bone remodeling, adaptation repair. Several ...

Lund DK, Mouly V, Cornelison DD. MMP-14 is necessary but not sufficient for invasion of three-dimensional collagen by human muscle satellite cells. Am J Physiol Cell Physiol 307: C140–C149, 2014. First published June 4, 2014; doi:10.1152/ajpcell.00032.2014.—The twenty-five known matrix metalloproteases (MMPs) and their endogenous inhibitors, tissue inhibitors of metalloproteases (TIMPs), mediat...

Soluble collagen-like components in whole human serum and in serum fractions obtained after filtration on Sephadex G-zoo or after sucrose density gradient centrifugation were analyzed by micro-immunodiffusion and by immunoelectrophoresis. Antiserum to human skin collagen served as the reagent for the detection of the antigen. The collagen-like components were shown to exist in at least z, and p...

Human umbilical endothelial cells (ECs) were grown on fibrillar type I collagen in 16.4-mm multiwell tissue culture plates. Human platelets were added to the wells, and platelet adhesion to collagen was examined by scanning electron microscopy and radioisotopic technique in the absence of ECs and in preconfluent and confluent EC cultures. Single adherent platelets of different shapes as well as...

A bacterial collagen-like protein Scl2 has been developed as a recombinant collagen model system to host human collagen ligand-binding sequences, with the goal of generating biomaterials with selective collagen bioactivities. Defined binding sites in human collagen for integrins, fibronectin, heparin, and MMP-1 have been introduced into the triple-helical domain of the bacterial collagen and le...

PURPOSE Collagen gels may prove to be potential carriers for transplantation of cultured corneal epithelial cells. The purpose of this study was to evaluate the suitability of collagen gels in comparison with corneal stromal blocks as the substrate to support the growth of human corneal epithelial cells in culture and the synthesis and deposition of the basement membrane components by these cel...

Objective(s):The aim of this study was to compare the chondrogenic induction potential of Piascledine and TGF-β1 on adipose-derived stem cells (ADSCs) in fibrin and fibrin-alginate scaffolds.  Materials and Methods: Human subcutaneous adipose tissues were harvested from three patients who were scheduled to undergo liposuction. Isolated ADSCs were proliferated in a culture medium. Then, the cell...